National Wildlife Health Center

Pathology and bacteriology of 178 tadpoles with histologically confirmed Severe Perkinsea Infections: Data

(i) This dataset presents macroscopic and microscopic pathological findings and bacteriology of 178 tadpoles diagnosed with Severe Perkinsea Infections. (ii) Specimen were collected and submitted to the NWHC as part of mortality investigations and collection of specimen from apparently healthy populations as part of ongoing amphibian health monitoring. (iii) Necropsies and gross evaluation of carcasses were carried out under a dissecting microscope. (iv) Severe Perkinsea Infection was confirmed in each specimen by histological observation of pathological changes in tissues with presence of Perkinsea-like organisms in at least one organ, including brain, eyes, gastrointestinal tract, gills, heart, liver, lungs, kidney,...

Categories: Data; Types: Map Service, OGC WFS Layer, OGC WMS Layer, OGC WMS Service; Tags: USGS Science Data Catalog (SDC), Unites States of Ameica, amphibian, biota, case definition, All tags... emerging infectious disease, histopathy, pathogenesis, pathogenic Perkinsea Clade, ranavirus, tadpoles, Fewer tags

Susceptibility and antibody response of the laboratory model zebra finch (Taeniopygia guttata) to West Nile Virus: Data

The data set contains the results of experimental challenge of captive zebra finches with an American crow isolate of West Nile virus (WNV). Data include infectivity, mortality, viremia, oral shedding of virus, and serology for anti- WNV antibodies. Australian and Timor zebra finches were used in this study and both are useful as a laboratory model of an avian species with moderate susceptibility to WNV.

Categories: Data; Types: Citation; Tags: National Wildlife Health Center, USGS Science Data Catalog (SDC), West Nile virus, immunoassay, qRT-PCR, All tags... survival analysis, Fewer tags

Histopathological assessment of the digestive gland of a freshwater snail (Lymnaea stagnalis) exposed to uranium

This dataset consists of measurements of the digestive gland in the freshwater snail Lymnaea stagnalis orally exposed to uranium. The objective of the study was to assess the morphological effects of uranium on the snail digestive gland by light microscopy. Laboratory-cultured L stagnalis were exposed to either synthetic or natural solid phases of uranium and a subset (6 controls and 6 exposed) were euthanized and fixed in 10% neutral buffered formalin for histopathological assessment. Slides were processed and stained with hematoxalin and eosin as per standard procedure (Luna 1968). The size of each sample, the length and width of digestive gland tubules (5 random glands per sample) and height of digestive cells...

Categories: Data; Tags: Environmental Health, Lymnaea stagnalis, Madison, Wisconsin, USGS Science Data Catalog (SDC), Wildlife Biology, All tags... Wildlife Disease, digestive gland, uranium, Fewer tags

SPV field trials bait uptake data

These data were collected as part of a field trial to test the effectiveness of a sylvatic plague vaccine (see Rocke et al., 2017 for details). Vaccine and control plots were selected randomly from the available pairs at each location. Baits containing Rhodamine B, a biomarker, were distributed at each plot. At least 1 week and no more than 2 months post-baiting each year, local collaborators captured, marked, and sampled prairie dogs. Hair and whisker samples were collected from up to 50 unique prairie dogs from each plot each year. Sex, age, weight, and the identity of all current-year and prior-year recaptures were recorded for each captured animal. In the laboratory, hair/whiskers were assessed for the presence...

Categories: Data; Types: Map Service, OGC WFS Layer, OGC WMS Layer, OGC WMS Service; Tags: Arizona, Awapa Plateau, Buffalo Gap National Grasslands, Cedar City, Charles M. Russell National Wildlife Refuge, All tags... Colorado, Coyote Basin, Espee Ranch, Gunnison, Larimer, Lower Brule, Montana, Pitchfork Ranch, Rita Blanca National Grasslands, South Dakota, Texas, USGS Science Data Catalog (SDC), Utah, Wind Cave National Park, biota, health, prairie dogs, sylvatic plague vaccine, Fewer tags

In vitro expression, immunogenicity, and efficacy data from recombinant raccoon poxvirus-vectored rabies vaccine candidates tested in mice

This work is part of an experimental trial to develop and assess novel recombinant raccoonpox virus (RCN) rabies vaccines in the mouse model, for potential use in bats. Briefly, our research group previously developed a recombinant RCN vaccine candidate expressing a mosaic glycoprotein (MoG) gene that protected mice and big brown bats when challenged with rabies virus (RABV). We developed two new recombinant RCN candidates expressing MoG (RCN-tPA-MoG and RCN-SS-TD-MoG) with the aim of improving RCN-MoG. We assessed and compared in vitro expression, in vivo immunogenicity, and protective efficacy in vaccinated mice challenged intracerebrally with RABV. In this data set, we share results of immunofluorescence assay...

Categories: Data; Tags: A/J mice, National Wildlife Health Center, Madison, WI, RABV, USGS Science Data Catalog (SDC), University of Wisconsin-Madison, Madison, WI - Hanson Biomedical Sciences, All tags... Wildlife Disease, biota, rabies, rabies virus, raccoon poxvirus, recombinant, vaccine, Fewer tags