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Jon J Amberg

Supervisory Fish Biologist

Upper Midwest Environmental Sciences Center

Office Phone: 608-783-6322
Fax: 608-783-6066
ORCID: 0000-0002-8351-4861

2630 Fanta Reed Road
La Crosse , WI 54603

Supervisor: Mark P Gaikowski
Description of Work This spring (2014) we will measure Asian carp eDNA over time at a Missouri River site downstream of multiple spawning areas before and during spawning. We will measure the amount of Asian carp eDNA in water samples. The amount of eDNA will be related to the numbers of eggs and larvae counted in matched samples (water samples taken at the same time and place). The time since the eDNA was released by the carp will be estimated and these results will be related to the average age of AC eggs and larvae in matched samples. We will also test for substances which interfere with the eDNA measurement techniques and can lead to false negatives. We will also be validating markers developed by USGS and...
Carbon dioxide (CO2) is under consideration as a chemical fish deterrent at pinch points in rivers (e.g., inside locks) to reduce unwanted fish passage. This study evaluated the behavioral responses of invasive round goby (Neogobius melanostomus) to dissolved CO2 in laboratory tanks. The goal of this project was to quantify CO2 concentrations that modify round goby behavior. More specifically, avoidance behavior (voluntary response) and loss of equilibrium (involuntary response) were quantified across 5, 15, and 25°C water temperatures. These data can be used to inform future field testing of CO2 as an invasive fish deterrent. Spreadsheets include data that were used to evaluate the efficacy of carbon dioxide (CO2)...
There is growing interest in the use of carbon dioxide (CO2) as a management tool for controlling invasive fishes. However, there is limited published data on susceptibility of many commonly encountered species to elevated CO2 concentrations. Our objective was to estimate the 24-h LC50 and LC95 of four fishes (Rainbow Trout Oncorhynchus mykiss, Common Carp Cyprinus Carpio, Channel Catfish Ictalurus punctatus, and Westslope Cutthroat Trout Oncorhynchus clarkii lewisi). In the laboratory, we exposed fingerlings to a range of levels of CO2 for 24-h in unpressurized, flow-through tanks. A Bayesian hierarchical model was developed to estimate the dose response relationship for each species of fish with associated uncertainty,...
Large amounts of data are being generated that require hours, days, or even weeks to analyze using traditional computing resources. Innovative solutions must be implemented to analyze the data in a reasonable timeframe. The program HTCondor ( takes advantage of the processing capacity of individual desktop computers and dedicated computing resources as a single, unified pool. This unified pool of computing resources allows HTCondor to quickly process large amounts of data by breaking the data into smaller tasks distributed across many computers. This project team implemented HTCondor at the USGS Upper Midwest Environmental Sciences Center (UMESC) to leverage existing computing...
This dataset includes information from ichthyoplankton sampling for silver carp and bighead carp collected from three main reaches in the Upper Mississippi River Basin, including the La Grange, Peoria, Starved Rock, and Marseilles reaches of the Illinois River, Pool 20 of Mississippi River, and the St. Croix River. Data include the number of bigheaded carp eggs and larvae identified using traditional manual sorting techniques, and genetic data derived from quantitative PCR (qPCR) and 4 bigheaded carp markers (SCTM4/5 and BHTM1/2) that were used to identify the amount of DNA in ichthyoplankton tow samples. These data provide information for natural resource agencies and managers who could use this qPCR screening...
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