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Person

Deborah Iwanowicz

Program Specialist

Office of the Associate Director for Ecosystems

Email: diwanowicz@usgs.gov
ORCID: 0000-0002-9613-8594

Location
LRL - Fish Health Lab (205)
11649 Leetown Road
Kearneysville , WV 25430
US

Supervisor: Rip S Shively
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The rusty-patched bumble bee (Bombus affinis) is a listed species under the Endangered Species Act of the United States. While understood to be a highly generalist forager, little is known on the role that limited floral resources or shifting floral community composition could have played in B. affinis decline. Determining which floral species provide suitable B. affinis forage could assist conservation efforts where B. affinis persists, identify floral species for restoration efforts, or highlight regions to search for cryptic populations.
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To identify potential biomarkers of salt stress in the freshwater mussel Elliptio complanata, we examined transcriptional responses of the common mussel Elliptio complanata to controlled NaCl exposures. The data set is a table of counts, with the rows being the transcripts detected in the samples in the experiment and the columns identifying the samples. Four mussles were exposed to normal freshwater and four mussels were exposed to high salinity (two ppt), for a period of seven days.
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Data were collected as part of an investigation developed by Leetown Science Center to investigate the comparative detection of avian influenza viruses from waterfowl and potential environmental reservoirs such as aquatic sediment from waterfowl habitat. This dataset identifies positive or negative test results for qRT-PCR (quantitative reverse transcriptase polymerase chain reaction) for avian influenza virus through identification of the Type A influenza virus matrix gene from aquatic sediment samples. Sediment samples were collected from waterfowl habitat so as to determine if temporal and spatial differences in virus detection by qRT-PCR were evident among test sites.
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Lake Sinai Viruses (LSV) are common ribonucleic acid (RNA) viruses of honey bees (Apis mellifera) that frequently reach high abundance but are not linked to overt disease. LSVs are genetically heterogeneous and collectively widespread, but despite frequent detection in surveys, the ecological and geographic factors structuring their distribution in A. mellifera are not understood. Even less is known about their distribution in other species. Better understanding of LSV prevalence and ecology have been hampered by high sequence diversity within the LSV clade. We developed a new genetic assay that detects all currently known lineages. We also performed pilot metagenetic sequencing to quantify the diversity of LSV...
Wastewater-based epidemiology (WBE) is used to determine the consumption of, or exposure to, chemicals or pathogens in human populations, and is conducted by collecting representative samples of untreated wastewater (influent) to quantify pathogens shed in the population served by the sampled wastewater system. The Centers for Disease Control and Prevention (CDC) and the US Department of Health and Human Services (HHS), in collaboration with the United States Geological Survey (USGS) and other agencies throughout the federal government, initiated the National Wastewater Surveillance System (NWSS) in response to the COVID-19 pandemic to conduct WBE using analytical methods for the collection and analysis of SARS-CoV-2...
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