Environmental DNA (eDNA) detection is a rapidly expanding technique used to non-invasively detect cryptic, low density, or logistically difficult-to-study species, such as imperiled manatees. Genetic material shed into the environment through tissue and body fluids is concentrated from water samples and analyzed for the presence of targeted eDNA. To help delineate manatee habitat ranges, high use areas, and seasonal population changes, a cytochrome-b quantitative PCR and state-of-the-art droplet digital PCR (ddPCR) eDNA assay was developed for the three extant and vulnerable manatee species: both subspecies of the West Indian manatee (Florida and Antillean), the African manatee and Amazonian manatee. Occurrence...
Categories: Data;
Types: Citation;
Tags: Cameroon,
Cuba,
Ecology,
Genetics,
Gulf of Mexico, All tags...
Trichechus,
USGS Science Data Catalog (SDC),
United States,
Wildlife Biology,
environmental DNA,
marine mammals,
multi-scale occupancy model,
non-invasive detection,
quantitative PCR, Fewer tags
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