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Varun Saraswathula

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In order to determine the innate microbial community of shale gas reservoirs and how they are impacted by hydraulic fracturing, this study analyzed biomass collected from produced water and rock from hydraulically fractured wells in the Utica Shale. The samples include rock chips from a drill core from one Utica well, produced water from that same Utica well, and produced water from 12 different Utica wells that had been in production between 1-5 years, spanning the oil and gas windows of SE Ohio. The samples were filtered for biomass, extracted, amplified, and 16S rRNA gene sequencing was performed on the Illumina MiSeq platform.
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Note: this data release has been superseded by a new version 3.0 published in 2023. Find the new version here: https://doi.org/10.5066/P9DSRCZJ. During hydrocarbon production, water is typically co-produced from the geologic formations producing oil and gas. Understanding the composition of these produced waters is important to help investigate the regional hydrogeology, the source of the water, the efficacy of water treatment and disposal plans, potential economic benefits of mineral commodities in the fluids, and the safety of potential sources of drinking or agricultural water. The U.S. Geological Survey National Produced Waters Geochemical Database v2.3 is an updated compilation of geochemical and related information...
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