Pacific Island Ecosystems Research Center
Email:
kfezzaroy@usgs.gov
Office Phone:
808-985-6437
ORCID:
0000-0002-7993-9031
Supervisor:
Robert N Reed
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We designed two new samplers for monitoring airborne particulates, including fungal and fern spores and plant pollen, that rely on natural wind currents (Passive Environmental Sampler) or a battery operated fan (Active Environmental Sampler). Both samplers are modeled after commercial devices such as the Rotorod® and the Burkard samplers, but are more economical and require less maintenance than commercial devices. We conducted wind tunnel comparisons of our two new samplers to Rotorod® samplers using synthetic polyethylene spheres (12 - 160 µm in diameter) to compare numbers and size range of particulates that are captured by the samplers. This dataset contains raw numbers of polyethylene spheres that were captured...
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These data include Ceratocystis culture viability results from ambrosia beetle (Coleopetera: Scolytinae) frass. Frass is defined as fine particles of macerated wood or boring dust, beetle parts, and feces. Frass was collected from individual ambrosia beetle galleries in Ceratocystis-infected ʻōhiʻa (Metrosideros polymorpha) trees on Hawaiʻi Island, a phenomenon referred to as Rapid ʻŌhiʻa Death (ROD). We recorded the height at which these beetles produced frass, how long we collected frass from galleries, and the total number of culturing tests from individual galleries.
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These data contain the raw quantitative polymerase chain reaction (qPCR) results for all Ceratocystis lukuohia and huliohia testing of ʻōhiʻa trees, soil, and seedlings.
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Rapid ʽŌhiʽa Death (ROD) currently threatens ōhiʽa lehua (Metrosideros polymorpha) on Hawaiʽi Island. First identified in Puna in 2014, the disease has now spread island wide. Besides direct sampling of trees, environmental sampling could serve as an easier and broader strategy to detect Ceratocystis spp., the fungi causing ROD. Environmental sampling could also help monitor the effect of felling ROD infected trees. We developed Passive and Active Environmental Samplers and deployed them at a property in Puna, where both C. lukuohia, and C. huliohia had been detected, and where the land owner practiced the management method of felling infected trees. We set up 2 Active Environmental Samplers (modified mosquito traps...
Categories: Data;
Tags: Ceratocystis,
Environmental DNA,
Environmental Sampler,
Hawaiii,
Management, All tags...
Metrosideros polymorpha,
Ohia lehua,
ROD,
Rapid Ohia Death,
Spore Traps,
environment, Fewer tags
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These data include the quantitative Polymerase Chain Reaction (qPCR) Ceratocystis lukuohia and C. huliohia DNA confirmations of viable subcultures. These data were used to determine the accuracy of morphological identifications.
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