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David M. Walters

This release is for data on Zinc concentrations and isotopic signatures of an aquatic insect (mayfly, Baetis tricaudatus). Mayflies were exposed to an aqueous zinc concentration gradient in a laboratory experiment. Zinc concentrations were measured in water, algae (mayfly food), and different mayfly lifestages. Natural abundances of carbon and nitrogen isotopes were also measured in different life stages. This data set includes on Zinc data. Isotope data are provided in a separate file. The abstract for a journal article explaining the results of the experiment follows below: Insect metamorphosis often results in substantial chemical changes that can fractionate isotopes and alter contaminant concentrations. We...
This release is for data on Zinc concentrations and isotopic signatures of an aquatic insect (mayfly, Baetis tricaudatus). Mayflies were exposed to an aqueous zinc concentration gradient in a laboratory experiment. Zinc concentrations were measured in water, algae (mayfly food), and different mayfly lifestages. Natural abundances of carbon and nitrogen isotopes were also measured in different life stages. This data set includes on Zinc data. Isotope data are provided in a separate file. The abstract for a journal article explaining the results of the experiment follows below: Insect metamorphosis often results in substantial chemical changes that can fractionate isotopes and alter contaminant concentrations. We...
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We measured polychlorinated biphenyl (PCB) concentrations in two taxa of riparian spiders from nine sites in 2011 at the Ashtabula River Area of Concern. We analyzed the extracts for 122 congeners following EPA method SW846 Method 8270D using gas chromatography mass spectrometry in the selective ion monitoring (SIM) mode. All target compounds were quantified using the method of internal standards, and all results were reported in ng/g (parts per billion) wet weight. Congener concentrations were summed to arithmetically calculate totals for individual homolog groups (groups of congeners that share the same number of chlorine atoms on the biphenyl ring) and for total PCBs. The samples were also analyzed for lipid...
Insect metamorphosis often results in substantial chemical changes that can fractionate isotopes and alter contaminant concentrations. We exposed larval mayflies (Baetis tricaudatus) to an aqueous zinc gradient (3-340 µg Zn/l) and measured the change in zinc tissue concentrations at different stages of metamorphosis. We also measured changes in stable isotopes (δ15N and δ13C) in unexposed B. tricaudatus. Zinc concentrations in larvae were positively related to aqueous zinc, increasing 9-fold across the exposure gradient. Zinc concentrations in adults were also positively related to aqueous concentrations, but were 7-fold lower than larvae. However, this relationship varied according to adult substage (subimago vs...
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