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The data support a study that describes the development and validation of a primer and probe based quantitative PCR (qPCR) assay for use with environmental DNA to detect Northwest salamander (Ambystoma gracile), a species endemic to the temperate Pacific coastal region of North America. The metadata includes qPCR quantification cycle (Cq) values from testing the A. gracile assay on DNA extracted from tissue samples derived from several A. gracile and closely related species and Cq values from testing the assay on environmental DNA (eDNA ) samples collected from two lakes, one containing A. gracile and one without the species.
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We collected environmental DNA (eDNA) data from the Elwha River, home to the world’s largest dam removal project, to track the spatial and temporal patterns of species responses following dam removal. In total, we collected data for 11 different fish taxa, sampled at 25 sites ranging across 56 river kilometers in a wilderness river for 4 years following dam removal. We show that eDNA can effectively be used to determine whether fish have recolonized past former dams, and in some cases determine the spatial extent of that recolonization.
Categories: Data;
Tags: Aquatic Biology,
Charr,
Dam Removal,
Ecology,
Elwha River, All tags...
Elwha River,
Genetics,
Salmon,
USGS Science Data Catalog (SDC),
biota,
environmental DNA, Fewer tags
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This release includes data used to evaluate the structure and function of the Ross Lake and Diablo Lake food webs. This includes data on zooplankton density and production (zooplankton_density.csv and daphnia_region_production_biomass.csv), lake volume estimates used to expand zooplankton density and production data (lake_volume_estimates.csv), fish sampling (FishSampleEvents.csv), fish biological information including diets, age, and stable isotope analysis (FishFullData_formatted.csv, FishPreyLength.csv), scale back-calculations (salmonid_back_calc.csv, rss_back_calc.csv), fish energy density (calorimetry_processed.csv), stable isotope data for invertebrate end members (si_inverts.csv), and hydroacoustic sampling...
Categories: Data;
Tags: Aquatic Biology,
Ecology,
Limnology,
Ross Lake National Recreation Area,
USGS Science Data Catalog (SDC), All tags...
bioenergetics,
biota,
fish diet,
food web,
freshwater ecosystems,
hydroacoustics,
inlandWaters,
invasive species,
zooplankton, Fewer tags
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Columns of data representing Chehalis River tributary, sample reach locations, dates of water sampling, quantitative PCR results, and whether lamprey were sampled by electrofishing in each sample reach. ***Please note that the title of this data release used to be "Environmental DNA surveys for lampreys in Chehalis River tributaries, Washington, from 2015 and 2017," but was updated for version 2.0.
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