Canyon riparian zone vegetation is vulnerable to effects of upstream river regulation. We studied box elder (Acer negundo) dominated canyon riparian forests intensively on the Green and Yampa rivers in Dinosaur National Monument, Colorado, and extensively in four other major rivers of the upper Colorado River Basin to determine the effects of river regulation on riparian tree establishment patterns. We: 1) aged individuals to determine if establishment was correlated with high annual peak flows, 2) mapped cohorts to determine if the areal extent of post-regulation cohorts was reduced on regulated compared to unregulated river reaches, and 3) measured the floodplain position of cohorts in regulated and unregulated rivers to determine [...]