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Assessing humoral and cell-mediated immune response in Hawaiian green turtles, Chelonia mydas

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Assessing humoral and cell-mediated immune response in Hawaiian green turtles, Chelonia mydas; 2000; Article; Journal; Veterinary Immunology and Immunopathology; Work, T. M.; Balazs, G. H.; Rameyer, R. A.; Chang, S. P.; Berestecky, J.

Summary

Seven immature green turtles, Chelonia mydas, captured from Kaneohe Bay on the island of Oahu were used to evaluate methods for assessing their immune response. Two turtles each were immunized intramuscularly with egg white lysozyme (EWL) in Freund’s complete adjuvant, Gerbu, or ISA-70; a seventh turtle was immunized with saline only and served as a control. Humoral immune response was measured with an indirect enzyme linked immunosorbent assay (ELISA). Cell-mediated immune response was measured using in vitro cell proliferation assays (CPA) using whole blood or peripheral blood mononuclear cells (PBM) cultured with concanavalin A (ConA), phytohaemagglutinin (PHA), or soluble egg EWL antigen. All turtles, except for one immunized with [...]

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Harvested on Tue Jun 30 04:16:00 MDT 2015 from MODS XML Service

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Type Scheme Key
local-index unknown 1003699
local-pk unknown 1003699
doi http://www.loc.gov/standards/mods/mods-outline-3-5.html#identifier doi:10.1016/S0165-2427(00)00168-9
series unknown Veterinary Immunology and Immunopathology

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journalVeterinary Immunology and Immunopathology
parts
typevolume
value74
typeissue
value3-4
languageEnglish
citationTypeArticle

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