Biofuels could reduce reliance on fossil oil, while helping to reduce greenhouse gas emissions and promoting rural development. This study assessed the viability of using local biodiesel production from sunflower in Tuscany (Italy) to meet inland demand for diesel fuel in compliance with the European Directives. A crop growth model, GIS and geostatistics were used to identify suitable areas for biodiesel production, considering potential sunflower yields alongside essential sustainability criteria: energy efficiency and greenhouse gas (GHG) savings throughout the supply chain. Simulation results indicate that biodiesel potential, estimated at 95,000 t/year, corresponds to 104,400 tCO2 eq/year of GHG saved and to 26,500 TOE/year of [...]