Agricultural losses of phosphorus (P) in runoff are a primary cause of eutrophication in many freshwater systems. A modified version of the P Index originally developed jointly by the USDA (Agricultural Research Service [ARS], Cooperative State Research, Education, and Extension Service [CSREES], and Natural Resources Conservation Service [NRCS]) was used to prioritize P loss vulnerability at the regional scale from 60 watersheds located within Minnesota using readily available data related to the transport and sources of P. This modified version of the P Index was created for a regionally based analysis of the index. Validation of the P Index rating was conducted using long-term water quality monitoring data consisting of total P [...]