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Emerging Methods for Detection and their Potential for Inventorying Brook Trout Populations in Streams of the Western Adirondacks

Dates

Start Date
2015-01-01
End Date
2017-10-01

Summary

Background Deoxyribonucleic acid (DNA) is the hereditary material in organisms that contains the biological instructions for building and maintaining them. The chemical structure of DNA is the same for all organisms, but differences exist in the order of the DNA building blocks, known as base pairs. Unique sequences provide a means to identify individual species and detect their presence within aquatic or terrestrial environments. Environmental DNA (eDNA) is nuclear or mitochondrial DNA that is shed from an organism into the environment. Sources of eDNA include feces, mucous, and gametes; shed skin; and carcasses. In aquatic environments, eDNA is diluted and distributed by currents and other hydrological processes. It may remain in [...]

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LK00-FXP01-Fig1.gif
“The structure of part of a DNA double helix”
thumbnail 982.02 KB image/gif
LK00-FXP01-Fig2.jpg
“map of the Adirondack Park, NY”
thumbnail 283.81 KB image/jpeg
LK00-FXP01-Fig3.png
“brook trout”
thumbnail 88.52 KB image/png
Shapefile: basin.zip
basin.shp 179.51 KB
basin.dbf 207 Bytes
basin.shx 108 Bytes
basin.prj 424 Bytes

Project Extension

parts
typeShort Project Description
valueAssess and refine eDNA methods for detecting the presence or absence (and relative abundance) of eastern brook trout and (b) determine the present-day status, relative abundance, and distribution of brook trout in headwater streams of the western Adirondacks. A related goal of this study is to assess the effectiveness (cost and time) of eDNA as a standardized tool for rapid and cost-effective collection of data that describes the status and distribution of brook trout in the Adirondacks.
projectProducts
productDescriptionThe USGS and AWI will compile and summarize project results in a final report or journal article that (1) defines the eDNA methodology and efficacy for detecting the presence or absence, and relative abundance, of eastern brook trout, (2) assesses how accurately eDNA characterizes the present-day status, relative abundance, and distribution of brook trout in headwater streams (compared to routine electrofishing surveys), and (3) evaluates the actual and potential effectiveness (cost and effort) of eDNA as tool to monitor and assess the health and distribution of brook trout (and other fish species) populations in streams of the western Adirondacks. The paper or report will go through internal technical and editorial reviews, be revised, and then be submitted to a peer-reviewed journal or published as a NYSERDA report. In addition, we will prepare a brief summary paper, approximately four to eight pages in length, which translates scientific findings into a fashion that is interesting, understandable, and appealing to a broad audience, including policy analysts, policy makers, and the interested general public.
statusDelivered
projectStatusCompleted

Additional Information

Alternate Titles

  • Brook Trout eDNA

Identifiers

Type Scheme Key
proposalno NY NY15-E
projectId BASIS+ LK00-FXP01

Shapefile Extension

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