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Retention of infectious haematopoietic necrosis virus infectivity in fish tissue homogenates and fluids stored at three temperatures.

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J. Burke, and D. Mulcahy, 1983, Retention of infectious haematopoietic necrosis virus infectivity in fish tissue homogenates and fluids stored at three temperatures.: Journal of Fish Diseases, v. 6, iss. 6.

Summary

Pools of brain, kidney, spleen, liver and gut tissues from several rainbow trout, Salmo gairdneri Richardson, and whole sockeye salmon, Oncorhynchus nerka (Walbaum), fry were homogenized with a known amount of infectious haematopoietic necrosis virus (IHNV). Virus was also added to ovarian fluids and sera pooled from several rainbow trout. The plaque assay was used to determine the retention of IHNV infectivity after different storage periods at 20°C, 4°C and —20°C. The work was used to evaluate homogenization as a remote field treatment of IHNV samples before shipment to the laboratory. Maintenance of viral infectivity varied widely among different homogenates and fluids. For short-term storage, 4°C was generally the most efficient [...]

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Author :
J. Burke, D. Mulcahy
Publisher :
Academic Press

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Harvested on Sun Jan 31 04:15:24 MST 2016 from MODS XML Service

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Type Scheme Key
local-index unknown 70162181
local-pk unknown 70162181
doi http://www.loc.gov/standards/mods/mods-outline-3-5.html#identifier doi:10.1111/j.1365-2761.1983.tb00109.x
series unknown Journal of Fish Diseases

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journalJournal of Fish Diseases
parts
typevolume
value6
typeissue
value6
languageEnglish
citationTypeArticle

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