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Assessment of environmental DNA for detecting presence of imperiled aquatic amphibian species in isolated wetlands

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McKee, A.M., Calhoun, D.L., Barichivich, W.J., Spear, S.F., Goldberg, C.S., and Glenn, T.C., 2015, Assessment of environmental DNA for detecting presence of imperiled aquatic amphibian species in isolated wetlands: Journal of Fish and Wildlife Management, v. 6, no. 2, p. 498-510, http://dx.doi.org/10.3996/042014-JFWM-034.

Summary

Environmental DNA (eDNA) is an emerging tool that allows low-impact sampling for aquatic species by isolating DNA from water samples and screening for DNA sequences specific to species of interest. However, researchers have not tested this method in naturally acidic wetlands that provide breeding habitat for a number of imperiled species, including the frosted salamander (Ambystoma cingulatum), reticulated flatwoods salamanders (Ambystoma bishopi), striped newt (Notophthalmus perstriatus), and gopher frog (Lithobates capito). Our objectives for this study were to develop and optimize eDNA survey protocols and assays to complement and enhance capture-based survey methods for these amphibian species. We collected three or more water [...]

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Type Scheme Key
local-index unknown 70168396
local-pk unknown 70168396
doi http://www.loc.gov/standards/mods/mods-outline-3-5.html#identifier doi:10.3996/042014-JFWM-034
series unknown Journal of Fish and Wildlife Management

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journalJournal of Fish and Wildlife Management
parts
typevolume
value6
typeissue
value2
languageEnglish
citationTypeArticle

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