Skip to main content
USGS - science for a changing world

Development of a dual luciferase-fluorescamine assay adapted to a 384 micro-well plate format-Data

Dates

Publication Date
Time Period
2016-09-16
Time Period
2016-10-14

Citation

Brennan, J.C., 2017, Development of a dual luciferase-fluorescamine assay adapted to a 384 micro-well plate format-Data: U.S. Geological Survey data release, http://dx.doi.org/10.5066/F7DV1H2F.

Summary

We have adapted two commonly used human breast luciferase transactivation cell bioassays, the recently re-named estrogen agonist/antagonist screening VM7Luc4E2 cell bioassay (previously designated BG1Luc4E2) and the androgen/glucocorticoid screening MDA-kb2 cell bioassay, to 384-well formats for HTS of endocrine-active substances. This cost-saving adaptation includes a fast, accurate, and easy measurement of protein amount in each well via the fluorescamine assay (in the form of relative fluorescence units, RFU which are then converted to microgram protein using bovine serum albumin standards) with which to normalize luciferase activity (in the form of relative light units, RLU) of cell lysates without requiring any transfer of the [...]

Contacts

Point of Contact :
Jennifer C Brennan
Originator :
Jennifer C Brennan
Metadata Contact :
CERC Data Managers
Publisher :
U.S. Geological Survey
Distributor :
U.S. Geological Survey - ScienceBase
USGS Mission Area :
Ecosystems
SDC Data Owner :
Columbia Environmental Research Center

Attached Files

Click on title to download individual files attached to this item.

BSA Standard Data.csv 6.31 KB
Treatment Data.csv 80.88 KB
Metadata_Development of a dual luciferase-fluorescamine assay adapted to a 384 micro-well plate format-Data.xml
Original FGDC Metadata

View
41.01 KB

Purpose

To verify a normalization of relative light units to microgram protein in cell lysates since the researchers require a quantitative measure of cell health of cells seeded in 384-well plates. This method may be applicable by other researchers to a number of luciferase transactivation cell bioassays and is adaptable to 96- or 384-well plate format.

Map

Spatial Services

ScienceBase WMS

Communities

  • Chesapeake Bay Endocrine Disrupting Chemicals
  • Columbia Environmental Research Center (CERC)

Tags

Provenance

Data source
Input directly

Additional Information

Identifiers

Type Scheme Key
DOI https://www.sciencebase.gov/vocab/category/item/identifier doi:10.5066/F7DV1H2F

Item Actions

View Item as ...

Save Item as ...

View Item...