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Detection of Nanophyetus salmincola in water, snails and fish tissues by quantitative PCR

Dates

Start Date
2015
End Date
2017
Publication Date

Citation

Purcell, Maureen, 2017, Detection of Nanophyetus salmincola in water, snails and fish tissues by quantitative PCR: U.S. Geological Survey data release, https://doi.org/10.5066/F7FN14QN.

Summary

The data are used to support the development and validation of two quantitative polymerase chain reaction (qPCR) assays to detect the parasite Nanophyetus salmincola DNA in water samples and fish and snail tissues. The data link to a series of experiments that are described in the publications. Experiment 1 defines the linearity and detection limits of the assays. Experiment 2 assesses the utility of the assays to detect N. salminicola waterborne cercaria in one liter volumes of water. Experiment 4 assesses the detection of N. salminicola waterborne cercaria in small volumes (50 microliters). Experiments 5 and 6 assesses the within and between run repeatability of the assays. Experiment 7 assesses the diagnostic specificity of the [...]

Contacts

Point of Contact :
Maureen Purcell
Originator :
Maureen Purcell
Metadata Contact :
Maureen Purcell
Publisher :
U.S. Geological Survey
Distributor :
U.S. Geological Survey - ScienceBase
USGS Mission Area :
Ecosystems
SDC Data Owner :
Western Fisheries Research Center

Attached Files

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Purcell_Nanophyetus_Data_Release_2017_tab_delimited.txt 124.7 KB
2064ea21-a09d-4c63-8717-9226f1d881d7.xml
Original FGDC Metadata

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27.53 KB

Material Request Instructions

Contact Maureen Purcell; mpurcell@usgs.gov

Purpose

The data are used to support the development and validation of two quantitative polymerase chain reaction (qPCR) assays to detect N. salmincola DNA in water samples and fish and snail tissues.

Communities

  • USGS Data Release Products

Tags

Provenance

Data source
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Additional Information

Identifiers

Type Scheme Key
DOI https://www.sciencebase.gov/vocab/category/item/identifier doi:10.5066/F7FN14QN

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