Skip to main content
USGS - science for a changing world

Conventional and quantitative PCR assays for detecting Tetracapsuloides bryosalmonae in fish tissue and environmental DNA water samples

Dates

Publication Date
Date Collected
2017-03-05
Date Collected
2017-06-01

Citation

Hutchins, P.R., and Sepulveda, A.J., 2017, Conventional and quantitative PCR assays for detecting Tetracapsuloides bryosalmonae in fish tissue and environmental DNA water samples: U.S. Geological Survey data release, https://doi.org/10.5066/F7C53JDW.

Summary

We developed and validated conventional and quantitative real-time PCR assays for the detection of DNA from the myxozoan parasite Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in fish. Assays were tested on fish tissue and on field-collected water samples to assess diagnostic and environmental DNA capabilities. The specificity, sensitivity, and broad applicability of the present assays surpass previous methods for detecting T. bryosalmonae DNA from fish tissue and water samples.

Child Items (2)

Contacts

Point of Contact :
Adam J Sepulveda
Originator :
Patrick R Hutchins, Adam J Sepulveda
Metadata Contact :
Adam J Sepulveda
Publisher :
U.S. Geological Survey
Distributor :
GS ScienceBase
USGS Mission Area :
Ecosystems
SDC Data Owner :
Northern Rocky Mountain Science Center

Purpose

Proof of concept and validation study for conventional PCR assays

Map

Spatial Services

ScienceBase WMS

Communities

  • Northern Rocky Mountain Science Center

Tags

Provenance

Additional Information

Identifiers

Type Scheme Key
DOI https://www.sciencebase.gov/vocab/category/item/identifier doi:10.5066/F7C53JDW

Item Actions

View Item as ...

Save Item as ...

View Item...