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Manatee environmental DNA data, and associated attributes, collected from the United States, Cuba, and Cameroon, from 2013-2015

Dates

Publication Date
Start Date
2013-11
End Date
2015-09

Citation

Hunter, M.E., Meigs-Friend, G., Ferrante, J.A., Takoukam Kamla, A., Dorazio, R.M., Keith-Diagne, L., Luna, F., Lanyon, J.M., and Reid, J.P., 2018, Manatee environmental DNA data, and associated attributes, collected from the United States, Cuba, and Cameroon, from 2013-2015: U.S. Geological Survey data release, https://doi.org/10.5066/F76M35S6.

Summary

Environmental DNA (eDNA) detection is a rapidly expanding technique used to non-invasively detect cryptic, low density, or logistically difficult-to-study species, such as imperiled manatees. Genetic material shed into the environment through tissue and body fluids is concentrated from water samples and analyzed for the presence of targeted eDNA. To help delineate manatee habitat ranges, high use areas, and seasonal population changes, a cytochrome-b quantitative PCR and state-of-the-art droplet digital PCR (ddPCR) eDNA assay was developed for the three extant and vulnerable manatee species: both subspecies of the West Indian manatee (Florida and Antillean), the African manatee and Amazonian manatee. Occurrence (ψ) and detection (p) [...]

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Attached Files

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ddPCR data_combined2.csv 23.86 KB
manatee environmental DNA attribute data 2013 to 2015.csv 3.6 KB
manatee eDNA metadata.xml
Original FGDC Metadata

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22.02 KB

Purpose

Data were obtained to in order to assess a manatee environmental DNA assay we developed to be able to non-invasively detect the presence of all manatee species from water samples, to assist in informing future management decisions.

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Communities

  • USGS Data Release Products
  • USGS Wetland and Aquatic Research Center

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Additional Information

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Type Scheme Key
DOI https://www.sciencebase.gov/vocab/category/item/identifier doi:10.5066/F76M35S6

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