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  3. Quantitative PCR standard curve and transcript values for the Florida manatee (Trichechus manatus latirostris), derived from 2013-14 manatee health assessments in Crystal River and Titusville, Florida

Quantitative PCR standard curve and transcript values for the Florida manatee (Trichechus manatus latirostris), derived from 2013-14 manatee health assessments in Crystal River and Titusville, Florida

Dates

Publication Date
Start Date
2013-12-03
End Date
2014-01-31

Citation

Ferrante, J.A., Hunter, M.E., and Wellehan, J.F.X., 2018, Quantitative PCR standard curve and transcript values for the Florida manatee (Trichechus manatus latirostris), derived from 2013-14 manatee health assessments in Crystal River and Titusville, Florida: U.S. Geological Survey data release, https://doi.org/10.5066/F7TB163Q.

Summary

These data represent the standard curve values as well as measurements of cytokine and reference gene transcripts from blood samples collected from Florida manatees and quantified using quantitative PCR.

Contacts

Point of Contact :
Jason A Ferrante
Process Contact :
Jason A Ferrante
Originator :
Jason A Ferrante, Margaret E Hunter, Wellehan, J.F.X.
Metadata Contact :
Jason A Ferrante
USGS Mission Area :
Ecosystems
SDC Data Owner :
Wetland and Aquatic Research Center
Distributor :
U.S. Geological Survey - ScienceBase

Attached Files

Click on title to download individual files attached to this item.

ManateeCytoQPCR_JF_reconciled.xml
Original FGDC Metadata
View 		28.99 KB application/fgdc+xml
2017JAF_TMLcytokine_DataRelease2_NormalizedData_v2.csv 4.9 KB text/csv
2017JAF_TMLcytokine_DataRelease1_RawData_v2.csv 126.63 KB text/csv

Purpose

These data were collected as part of the validation and optimization of quantitative PCR assays which are to be used in the study of Florida manatee immune function. These data can be appropriately used to quantify transcript (mRNA) levels of target cytokines and reference genes in manatee blood.

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Type Scheme Key
DOI https://www.sciencebase.gov/vocab/category/item/identifier doi:10.5066/F7TB163Q

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