This report summarizes the results of a three-year investigation of terrestrial habitat connectivity priorities for the South Atlantic Landscape Conservation Cooperative (South Atlantic LCC). Our primary objective was to generate results that could be used to drive fine‐scaled conservation planning to enhance habitat connectivity across the South Atlantic LCC. The project focused on seven target species, including large mammals (black bear, red wolf, Florida panther/eastern cougar) and a group of terrestrial reptiles (eastern diamondback rattlesnake, timber rattlesnake, pine snake, and box turtle). We used two different modeling approaches to identify areas with either high predicted flow of a given species (Circuitscape) or greater importance to the overall habitat network for a given species (Connectivity Analysis Toolkit). We parameterized our models with a combination of expert opinion, landcover data, and traffic information, and we projected the same models into the future (year 2100) using predictions of urbanization and sea level rise.