Conservation planning and delivery is often carried out by multiple stakeholders in isolation and constrained to political boundaries. Developing a regional conservation blueprint through partnership is an important component of the effort to conserve biodiversity in the face of declining agency capacity (staff and budgets) and rapidly changing landscapes and climate. In 2012, Missouri, Oklahoma, and Arkansas committed to developing a shared vision for conservation in the Ozark Highlands ecological region. Staff from the Gulf Coastal Plains & Ozarks Landscape Conservation Cooperative and the Central Hardwoods Joint Venture engaged in this effort to provide geospatial and conservation planning capacity. The resulting products provide a preliminary conservation design that can be used to fulfill some of the requirements for State Wildlife Action Plan (a.k.a. Comprehensive Conservation Strategy) revision for the Ozark portion of the respective states. Further, the process provides the JV and LCC a partner-driven framework for conservation design and delivery that is transparent, replicable & defensible (i.e. scientific). This project embodies Strategic Habitat Conservation and sets the pace for conservation regionally and nationally by demonstrating how to develop a comprehensive plan/strategy by ecological unit that spans multiple states.