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Quantitative polymerase chain reaction (qPCR): An alternative, rapid water quality monitoring tool at a National Park on Lake Michigan.

Dates

Publication Date
Time Period
2014

Citation

U.S. Geological, Great Lakes Science Center, Lake Michigan Ecological Research Station, 2018, Quantitative polymerase chain reaction (qPCR): An alternative, rapid water quality monitoring tool at a National Park on Lake Michigan.: U.S. Geological Survey data release, https://doi.org/10.5066/F77P8XND.

Summary

Data were collected to evaluate the efficacy of quantitative polymerase chain reaction (qPCR) as a rapid, alternative method for monitoring recreational water at select beaches and rivers of Sleeping Bear Dunes National Lakeshore (SLBE), in Empire, Michigan. Water samples were collected between August 4 and September 18, 2014 (N=297) from four locations (Esch Rd, Otter Creek, Platte Bay, and Platte River). The samples were analyzed for indicator bacteria, E. coli and enterococci, by both culture-based (membrane filtration, MF; traditional method) and non-culture based (qPCR; rapid method). Recreational water quality standards (RWQS) and beach action values (BAV) were used as indices to compare water quality standard exceedances for [...]

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CultureBasedData.csv 36.47 KB
Lat_Long.csv 556 Bytes
qPCR_v2.csv 57.62 KB
SLBE data release_v4.xml
Original FGDC Metadata

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28.32 KB

Purpose

These data were collected to evaluate qPCR (DNA based method) as an alternative, rapid water quality-monitoring tool using E. coli and enterococci as fecal indicator bacteria.

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  • Great Lakes Science Center (GLSC)

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Type Scheme Key
DOI https://www.sciencebase.gov/vocab/category/item/identifier doi:10.5066/F77P8XND

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