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Establishing a standard ploidy assessment method using grass carp from Ohio, 2015-2016

Dates

Publication Date
Start Date
2015
End Date
2016

Citation

Jenkins, J.A., Johnson, D., and Kinter, B.T., 2018, Establishing a standard ploidy assessment method using grass carp from Ohio, 2015-2016: U.S. Geological Survey data release, https://doi.org/10.5066/F7QC02D4.

Summary

In 2015-2016, the Ohio Division of Wildlife’s undercover law enforcement purchased 1,200 grass carp (Ctenopharyngodon idella). Fish heads and eyeballs were sent overnight to U.S. Geological Survey Wetland and Aquatic Research Center for ploidy analysis. Field and laboratory standard operating procedures were established and followed. Fish lengths, fish weights, and eyeball weights were obtained from the U.S. Fish and Wildlife Service’s feral carp ploidy program for grass carp and black carp (Mylopharyngodon piceus) and the Ohio grass carp. Internal 2µm or 4µm bead standards were used in establishing nuclear sizes from Nile tilapia (Oreochromis niloticus), known diploid (n=20) and triploid (n=20) carp blood, as well as cells derived [...]

Contacts

Point of Contact :
Jill A Jenkins
Originator :
Jill A Jenkins, Darren Johnson, Bryan T Kinter
Metadata Contact :
Jill A Jenkins
Publisher :
U.S. Geological Survey
USGS Mission Area :
Ecosystems
SDC Data Owner :
Wetland and Aquatic Research Center
Distributor :
U.S. Geological Survey - ScienceBase

Attached Files

Click on title to download individual files attached to this item.

Ohio_BufferOsmolality.csv 1.9 KB
Ohio_CarpLengthsWeights.csv 1.44 KB
Ohio_ControlledExperiment.csv 7.78 KB
Ohio_FieldFlowCytometryData.csv 27.63 KB
Ohio_2015-2016_MasterLegend.xlsx 15.43 KB
Ohio_2015-2016_Metadata.xml
Original FGDC Metadata

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30.38 KB

Purpose

Data were obtained in order to establish a laboratory method for ploidy determination that can be used in a court of law for law enforcement purposes according to state compliance with their laws regarding diploid grass carp and black carp.

Map

Communities

  • USGS Data Release Products
  • USGS Wetland and Aquatic Research Center

Tags

Provenance

Additional Information

Identifiers

Type Scheme Key
DOI https://www.sciencebase.gov/vocab/category/item/identifier doi:10.5066/F7QC02D4

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