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Establishing a standard ploidy assessment method using grass carp from Ohio, 2015-2016


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Jenkins, J.A., Johnson, D., and Kinter, B.T., 2018, Establishing a standard ploidy assessment method using grass carp from Ohio, 2015-2016: U.S. Geological Survey data release,


In 2015-2016, the Ohio Division of Wildlife’s undercover law enforcement purchased 1,200 grass carp (Ctenopharyngodon idella). Fish heads and eyeballs were sent overnight to U.S. Geological Survey Wetland and Aquatic Research Center for ploidy analysis. Field and laboratory standard operating procedures were established and followed. Fish lengths, fish weights, and eyeball weights were obtained from the U.S. Fish and Wildlife Service’s feral carp ploidy program for grass carp and black carp (Mylopharyngodon piceus) and the Ohio grass carp. Internal 2µm or 4µm bead standards were used in establishing nuclear sizes from Nile tilapia (Oreochromis niloticus), known diploid (n=20) and triploid (n=20) carp blood, as well as cells derived [...]


Point of Contact :
Jill A Jenkins
Originator :
Jill A Jenkins, Darren Johnson, Bryan T Kinter
Metadata Contact :
Jill A Jenkins
Publisher :
U.S. Geological Survey
USGS Mission Area :
SDC Data Owner :
Wetland and Aquatic Research Center
Distributor :
U.S. Geological Survey - ScienceBase

Attached Files

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Ohio_BufferOsmolality.csv 1.9 KB
Ohio_CarpLengthsWeights.csv 1.44 KB
Ohio_ControlledExperiment.csv 7.78 KB
Ohio_FieldFlowCytometryData.csv 27.63 KB
Ohio_2015-2016_MasterLegend.xlsx 15.43 KB
Original FGDC Metadata

30.38 KB


Data were obtained in order to establish a laboratory method for ploidy determination that can be used in a court of law for law enforcement purposes according to state compliance with their laws regarding diploid grass carp and black carp.



  • USGS Data Release Products
  • USGS Wetland and Aquatic Research Center



Additional Information


Type Scheme Key
DOI doi:10.5066/F7QC02D4

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