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Ethanol and sodium acetate as a preservation method to delay degradation of environmental DNA: Data

Dates

Publication Date
Start Date
2014-03-04
End Date
2014-03-12

Citation

Bridget Ladell, Liza Walleser, Grace McCalla, Richard Erickson, and Jon Amberg, 2018, Ethanol and sodium acetate as a preservation method to delay degradation of environmental DNA: Data: U.S. Geological Survey data release, https://doi.org/10.5066/F7FB527Z.

Summary

This data contains environmental DNA (eDNA) sample assay results that were collected from water samples taken from a tank housing Asian carp and placed either on ice or at room temperature. At both treatment temperatures, water samples were left untreated or were treated with an ethanol and sodium acetate solution (EtOH-NaAc). This was done to evaluate an ethanol and sodium acetate solution to maintain the integrity of the DNA samples for the time between collection and lab testing. Every day for 6 days following collection, a subset of the samples was removed from each treatment and DNA was extracted and nuclear and mitochondrial markers were assayed with qPCR. Results showed comparable persistence of DNA between untreated iced samples [...]

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Attached Files

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Ethanol_and_sodium_acetate_as_a_preservation_method_to_delay_degradation_of_environmental_DNA.xml
Original FGDC Metadata

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25.56 KB
Mitochon_Marker.csv 3.41 KB
Nuclear_Marker.csv 3.44 KB

Purpose

The data were collected to determine if environmental DNA could still be detected by PCR after being stored in an ethanol/sodium acetate solution rather than the conventional storage on ice.

Communities

  • Upper Midwest Environmental Sciences Center (UMESC)

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