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Surveys of environmental DNA (eDNA): a new approach to estimate occurrence in Vulnerable manatee populations

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Hunter, M.E., Meigs-Friend, G., Ferrante, J.A., Takoukam Kamla, A., Dorazio, R.M., Keith Diagne, L., Luna, F., Lanyon, J.M., and Reid, J.P., 2018, Surveys of environmental DNA (eDNA): a new approach to estimate occurrence in vulnerable manatee populations: Endangered Species Research, v. 35, p. 101-111, https://doi.org/10.3354/esr00880.

Summary

Environmental DNA (eDNA) detection is a technique used to non-invasively detect cryptic, low density, or logistically difficult-to-study species, such as imperiled manatees. For eDNA measurement, genetic material shed into the environment is concentrated from water samples and analyzed for the presence of target species. Cytochrome bquantitative PCR and droplet digital PCR eDNA assays were developed for the 3 Vulnerable manatee species: African, Amazonian, and both subspecies of the West Indian (Florida and Antillean) manatee. Environmental DNA assays can help to delineate manatee habitat ranges, high use areas, and seasonal population changes. To validate the assay, water was analyzed from Florida’s east coast containing a high-density [...]

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Type Scheme Key
local-index unknown 70196591
local-pk unknown 70196591
doi http://www.loc.gov/standards/mods/mods-outline-3-5.html#identifier doi:10.3354/esr00880
series unknown Endangered Species Research

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journalEndangered Species Research
parts
typevolume
value35
languageEnglish
citationTypeArticle

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