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2015 environmental DNA surveys for lampreys in Chehalis River tributaries, Washington


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Ostberg, C.O., Chase, D.M., Hoy, M.S., Duda, J.J., Hayes, M.C., Jolley, J.C, Silver, G.S., Barkstedt, J., and Cook-Tabor, C., 2019, 2015 environmental DNA surveys for lampreys in Chehalis River tributaries, Washington (ver. 3.0, June 2019): U.S. Geological Survey data release,


Columns of data representing Chehalis River tributary, sample reach locations, dates of water sampling, quantitative PCR results, and whether lamprey were sampled by electrofishing in each sample reach. ***Please note that the title of this data release used to be "Environmental DNA surveys for lampreys in Chehalis River tributaries, Washington, from 2015 and 2017," but was updated for version 2.0.


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Ostberg_Chehalis lamprey eDNA data release_2018_vs 3.0.csv 47.31 KB
Data release revision history vs 3.0.txt 2.28 KB


The goal was to apply an occupancy-based study design with electrofishing and eDNA sampling methods to investigate Pacific Lamprey and Lampetra spp occupancy in tributaries of the Chehalis River, a Washington coastal river. Such information can be used to refine the occupancy-based sampling approach for Washington coastal streams and provide needed information on current distributions. There were three objectives for this research study. The first objective was to provide information on lamprey spatial distributions in the Chehalis River basin. The second objective was to empirically evaluate the efficacy of eDNA-based methods for detecting lamprey relative to electrofishing. The third objective was to analyze eDNA results using multiscale occupancy model to estimate the probability of eDNA occurrence at sample locations, in replicate water samples, and in replicate PCRs. In addition, the relationship between eDNA concentration and distance of sample location from the Chehalis River within tributaries was explored.



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Revision 2.0 by Carl Ostberg on March 6, 2019 and revision 3.0 by Carl Ostberg on June 10, 2019. To review the changes that were made, see “Data release revision history vs 3.0.txt” in the attached files section.

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DOI doi:10.5066/P9ZU4EU7

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