The transboundary region of Washington and British Columbia (Fig. 1) is important for the conservation of many wildlife species. Some species of conservation concern, such as wolverine (Gulo gulo) and Canada lynx (Lynx canadensis), have home ranges that span the international border. Other species depend on the region for seasonal habitat. All regional wildlife species will require a connected network of habitats spanning the border as they adjust their ranges to meet life history requirements under future changes to climate and land-use. Previous analyses have identified numerous areas within the region where habitat connectivity has been disrupted by human activities (Gaines et al. 2001; Singleton et al. 2002, 2004; WHCWG 2010) or by natural barriers to species movements. Particularly affected areas include the Fraser River-Coquihalla Valley, Okanagan Valley, Upper Columbia and Pend Oreille River valleys, and the various highway corridors that bisect the transboundary area. Fine-scale, operational analyses are urgently needed to guide on-the-ground conservation and management actions aimed at maintaining and restoring transboundary connectivity for wildlife.