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Droplet digital PCR data from method testing to improve eDNA yield and reduce inhibitors from environmental water samples

Dates

Publication Date
Start Date
2014-11-01
End Date
2018-09-25

Citation

Hunter, M.E., Ferrante, J.A., Meigs-Friend, G., and Ulmer, A.M., 2019, Droplet digital PCR data from method testing to improve eDNA yield and reduce inhibitors from environmental water samples: U.S. Geological Survey data release, https://doi.org/10.5066/P9ULOPXB.

Summary

Data files for manuscript "Improving eDNA yield and inhibitor reduction through increased water volumes and modified multi-filter isolation techniques". These include the results of different treatments for inhibitor removal from a water sample and DNA yield quantities from such tests. Environmental DNA (eDNA) methods detect genetic material that is shed into the environment through skin cells and excrement by plants and animals. These methods are increasingly being applied to detect imperiled and invasive species for range assessment and occurrence estimates. To improve the recovery and detection of eDNA, we tested and optimized three basic protocols to 1) assess whether eDNA concentration increase in a linear fashion, 2) test the [...]

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Attached Files

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Figure_1_DataRelease_R.csv
“Study 1 data”
4.98 KB
Figure_2_DataRelease_R.csv
“Study 2 data”
14.63 KB
Figure_3_DataRelease_R.csv
“Study 3 data”
10.45 KB
2018_IsoInhi_ms.xml
Original FGDC Metadata

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36.83 KB

Purpose

Methodological enhancements are needed to reduce filter clogging, PCR inhibition, and false-negative detections when environmental DNA (eDNA) is at low concentrations. These eDNA methods produce data that are used to inform management and conservation decisions.

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  • USGS Data Release Products
  • USGS Wetland and Aquatic Research Center

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DOI https://www.sciencebase.gov/vocab/category/item/identifier doi:10.5066/P9ULOPXB

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