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Quantification of nitrogen-fixing microorganisms (targeting nifH gene) in Cladophora algae by quantitative polymerase chain reaction (qPCR)

Dates

Publication Date
Start Date
2015-06-18
End Date
2015-09-23

Citation

Byappanahalli, M.N., Przybyla‐Kelly, K., Ishii, S., and Aunins, A.W., 2019, Quantification of nitrogen‐fixing microorganisms (targeting the nifH gene) in Cladophora algae by quantitative polymerase chain reaction (qPCR): U.S. Geological Survey data release, https ://doi.org/10.5066/P9U2SYRI.

Summary

Data were collected to determine the abundance of nitrogen-fixing microorganisms in Cladophora algae growing on rocks, breakwall structures, or submerged dreissenid mussel beds around southern Lake Michigan. Cladophora samples (N=33) were collected between June and September 2015 from three urban areas: (a) Jeorse Park, East Chicago, Indiana, (b) Calumet Beach, Chicago, Illinois, and (c) North Beach, Racine, Wisconsin, and a National Park site, Portage Lakefront, Indiana Dunes National Park, Indiana. Corresponding lake water samples (N=33) were collected approximately 15-20 feet away from submerged algal mats. Genomic DNA was extracted from water and processed algal samples. The abundance of nitrogen-fixing microorganisms in water [...]

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Attached Files

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Lat_Long.csv 516 Bytes
PowerBiofilm.pdf 699.02 KB
PowerWater.pdf 59.61 KB
NifH_qPCR.csv 4.65 KB
NifH_taxonomicID.csv 40.78 KB
NifH_v5.xml
Original FGDC Metadata

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30.33 KB

Purpose

The data were collected to determine the abundance of nitrogen-fixing microorganisms by targeting the nifH gene in actively growing (i.e., fresh) Cladophora algae in Lake Michigan. There is a significant knowledge gap in the abundance, taxonomic diversity, and nitrogen-fixing capabilities of these microorganisms. This information is critical for two reasons: first, to explain Cladophora’s nitrogen needs; second, to develop strategies to manage the nuisance algal problem by reducing nutrients entering the lakes via streams and other associated waterways.

Additional Information

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DOI https://www.sciencebase.gov/vocab/category/item/identifier doi:10.5066/P9U2SYRI

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