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Reporting the limits of detection (LOD) and quantification (LOQ) for environmental DNA assays: Data

Dates

Publication Date
Start Date
2017-11-01
End Date
2019-05-01

Citation

Merkes, C., Klymus, K., Allison, M., Goldberg, C., Helbin, C., Hunter, M., Jackson, C., Lance, R., Mangan, A., Monroe, E., Piagio, A., Stokdyk, J., Wilson, C., and Richter, C., 2019, Reporting the limits of detection (LOD) and quantification (LOQ) for environmental DNA assays: Data: U.S. Geological Survey data release, https://doi.org/10.5066/P9AKHU1R.

Summary

This data set was collected to provide examples and aid in developing a standardized way of determining LOD and LOQ for eDNA assays and has 3 data files. GEDWG_LOD_DATA3.csv is raw qPCR data from multiple labs running multiple standards of known concentration for eDNA assays they regularly use. Comparison-Data.csv is the merged data output from running a generic LOD/LOQ calculator script multiple times with different LOD model settings. The generic LOD/LOQ calculator script is available at: https://github.com/cmerkes/qPCR_LOD_Calc, and details about the multiple settings used are commented in the analysis script available at: https://github.com/cmerkes/LOD_Analysis

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Comparison-Data.csv 95.22 KB text/csv
GEDWG_LOD_DATA3.csv 450.75 KB text/csv
LODLOQ_AssayInfo_043019.csv 41.77 KB text/csv

Purpose

The data were collected to provide examples and aid in developing a standardized way of determining LOD and LOQ for eDNA assays.

Map

Communities

  • USGS Data Release Products
  • Upper Midwest Environmental Sciences Center (UMESC)

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Type Scheme Key
DOI https://www.sciencebase.gov/vocab/category/item/identifier doi:10.5066/P9AKHU1R

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