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  3. Acetylene Consumption and Dechlorination by a Groundwater Microbial Enrichment Culture

Acetylene Consumption and Dechlorination by a Groundwater Microbial Enrichment Culture

Dates

Publication Date
Start Date
2016-06
End Date
2019

Citation

Baesman, S.M., Akob, D.M., Gushgari-Doyle, S., Oremland, R., Keren, R., Banfield, J.F., and Alvarez-Cohen, L., 2020, Acetylene Consumption and Dechlorination by a Groundwater Microbial Enrichment Culture: U.S. Geological Survey data release, https://doi.org/10.5066/P9BF8LM4.

Summary

This study aimed to (1) enrich microbial acetylenotrophs from trichloroethylene (TCE) contaminated groundwater and (2) evaluate whether these enrichments could degrade TCE coupled to acetylene degradation. Acetylenotrophs are microorganisms that use acetylene as their carbon and energy source. TCE contaminated groundwater was collected from wells at the Naval Air Warfare Center (NAWC) in West Trenton, New Jersey. Microbial acetylene uptake in groundwater samples was established by mixing the groundwater with a defined mineral medium to supply nutrients and providing acetylene as the sole electron donor and carbon source. The structure of the microbial community in those enrichments was characterized as shown by 16S rRNA gene sequencing [...]

Contacts

Point of Contact :
Denise M Akob, Water Resources
Originator :
Shaun Baesman, Denise M Akob, Sara Gushgari-Doyle, Ronald S Oremland, Ray Keren, Jillian F. Banfield, Lisa Alvarez-Cohen
Metadata Contact :
Denise M Akob
Publisher :
U.S. Geological Survey
Distributor :
U.S. Geological Survey - ScienceBase
SDC Data Owner :
Laboratory & Analytical Services Division
USGS Mission Area :
Environmental Health

Attached Files

Click on title to download individual files attached to this item.

NAWC_Acetylene_metadata_2019_v10.xml
Original FGDC Metadata
View 		37.44 KB application/fgdc+xml
NAWC_C2H2_TCE_data_release.xlsx 31.19 KB application/vnd.openxmlformats-officedocument.spreadsheetml.sheet
Fig1_acetyleneTCE_data.csv 3.12 KB text/csv
Fig_S2_microbial_communities.csv 894 Bytes text/csv
Fig_S1_C2H2 uptake by NAWC GW.csv 7.47 KB text/csv

Purpose

The data collected were obtained to evaluate whether acetylene could fuel chlorinated solvent degradation by groundwater microbial communities. Microbial acetylenotrophs were enriched from trichloroethylene (TCE) contaminated groundwater then the enrichments were evaluate for their ability to degrade TCE or tetrachloroethene (PCE) coupled to acetylenotrophy. Microbial community characterization of the acetylenotrophic enrichments helped identify the microbial populations responsible for acetylenotrophic activity.

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Type Scheme Key
DOI https://www.sciencebase.gov/vocab/category/item/identifier doi:10.5066/P9BF8LM4

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