Identify sources of high E. coli concentrations, beaches of southern Lake Michigan, 2015 (version 2.0, July 2020)
Dates
Publication Date
2018-03-02
Start Date
2015-06-11
End Date
2015-09-04
Last Revision
2020-07-31
Citation
Nevers, M.B., Byappanahalli, M.N., and Shively, D.A., 2018, Identify sources of high E. coli concentrations, beaches of southern Lake Michigan, 2015 (version 2.0, July 2020): U.S. Geological Survey data release, https://doi.org/10.5066/F7H70F3D.
Summary
Data were collected as part of a study to identify sources of E. coli contamination at several beaches located in the Grand Calumet River Areas of Concern, located in northern Indiana on Lake Michigan, as well as in Illinois and Wisconsin on Lake Michigan. Water samples were collected at each site in Indiana three times a week for thirteen weeks and at each site in Illinois and Wisconsin. All samples were analyzed for E. coli bacteria (an indicator bacteria for fecal contamination) and species-specific molecular markers (microbial source tracking, MST), including human, gull, and dog. Presence of MST markers indicates a fecal source at that location associated with the target animal. Field conditions were recorded during each site [...]
Summary
Data were collected as part of a study to identify sources of E. coli contamination at several beaches located in the Grand Calumet River Areas of Concern, located in northern Indiana on Lake Michigan, as well as in Illinois and Wisconsin on Lake Michigan. Water samples were collected at each site in Indiana three times a week for thirteen weeks and at each site in Illinois and Wisconsin. All samples were analyzed for E. coli bacteria (an indicator bacteria for fecal contamination) and species-specific molecular markers (microbial source tracking, MST), including human, gull, and dog. Presence of MST markers indicates a fecal source at that location associated with the target animal. Field conditions were recorded during each site visit (e.g., including air and water temp, wind speed and direction, rainfall, wave height, currents, observations). Water samples were analyzed in the laboratory for E. coli using defined substrate technology and for MST markers using quantitative polymerase chain reaction (qPCR) methods and also turbidity. Lat_long_v2 file includes information regarding sampling locations and their corresponding latitude and longitudes. The E coli_turbidity_v2 data include E. coli densities and turbidity measurements. Data from the qpcr_v4 file includes results from quantitative polymerase chain reaction (qPCR) method for detection of host-specific microbial source tracking markers. Data from SanitarySurvey includes ambient conditions measured in the field: water and air temperature, current speed and direction (eastward, westward, float method), wind direction and speed, wave height, rainfall, and cloud cover. Data from SanitarySurvey_birds included number of birds (gulls, geese, ducks, cormorants, etc.) counted on the beach and in the water. The PowerWater.pdf is the DNA extraction kits' user manual giving detailed instructions for use.
Click on title to download individual files attached to this item.
MST data release_v3-2.xml Original FGDC Metadata
View
57.15 KB
application/fgdc+xml
Lat_Long_v2.csv
752 Bytes
text/csv
PowerWater.pdf
264.3 KB
application/pdf
E coli_turbidity_v2.csv
21.2 KB
text/csv
qpcr_v4.csv
121.43 KB
text/csv
SanitarySurvey_birds.csv
6.17 KB
text/csv
SanitarySurvey.csv
27.31 KB
text/csv
MST data release_v3-1.txt
764 Bytes
text/plain
Related External Resources
Type: Related Primary Publication
Nevers, M.B., Byappanahalli, M.N., Shively, D., Buszka, P.M., Jackson, P.R., and Phanikumar, M.S., 2018, Identifying and Eliminating Sources of Recreational Water Quality Degradation along an Urban Coast: Journal of Environmental Quality, v. 47, no. 5, p. 1042–1050, https://doi.org/10.2134/jeq2017.11.0461.
These data were collected to examine potential sources of high concentrations of E. coli at shoreline locations within the Grand Calumet River Area of Concern (Indiana) and Illinois and Wisconsin using host-specific, DNA-based molecular markers. Additionally, at AOC locations, modified sanitary surveys were used; data were also used to evaluate the effectiveness of a gull deterrence program using trained canines.
