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Genetic detection of Lake Sinai Virus in honey bees (Apis mellifera) and other insects

Dates

Publication Date
Start Date
2017-01-01
End Date
2019-12-31

Citation

Cornman, R.S., Iwanowicz, D.I., and Otto, C.R.V., 2020, Genetic detection of Lake Sinai Virus in honey bees (Apis mellifera) and other insects: U.S. Geological Survey data release, https://doi.org/10.5066/P9O9ZMA1.

Summary

Lake Sinai Viruses (LSV) are common ribonucleic acid (RNA) viruses of honey bees (Apis mellifera) that frequently reach high abundance but are not linked to overt disease. LSVs are genetically heterogeneous and collectively widespread, but despite frequent detection in surveys, the ecological and geographic factors structuring their distribution in A. mellifera are not understood. Even less is known about their distribution in other species. Better understanding of LSV prevalence and ecology have been hampered by high sequence diversity within the LSV clade. We developed a new genetic assay that detects all currently known lineages. We also performed pilot metagenetic sequencing to quantify the diversity of LSV sequences. The resulting [...]

Contacts

Point of Contact :
Robert S Cornman
Originator :
Robert S Cornman, Deborah Iwanowicz, Clint R Otto
Metadata Contact :
Robert S Cornman
Publisher :
U.S. Geological Survey
Distributor :
U.S. Geological Survey - ScienceBase
SDC Data Owner :
Fort Collins Science Center
USGS Mission Area :
Ecosystems

Attached Files

Click on title to download individual files attached to this item.

detection.rates.txt 1.53 KB text/plain
LSV.biom.txt 90.99 KB text/plain
lsv.biosamples.txt 1.81 KB text/plain
LSV.OTU.peptide.FASTA 73.24 KB text/plain

Purpose

The data were collected to validate a new genetic test for Lake Sinai Viruses (LSV) in insects. The data may be used as evidence of the strains of LSV that may be present in insect hosts.

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Communities

  • Fort Collins Science Center (FORT)
  • USGS Data Release Products

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Additional Information

Identifiers

Type Scheme Key
DOI https://www.sciencebase.gov/vocab/category/item/identifier doi:10.5066/P9O9ZMA1

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