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Abundance of 50 transcripts from microdissected liver samples of smallmouth bass


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Walsh, H.L., Blazer, V.S., and Sperry, A.J., 2020, Abundance of 50 transcripts from microdissected liver samples of smallmouth bass: U.S. Geological Survey data release,


There is an increasing emphasis on effects-based monitoring to document responses associated with exposure to complex mixtures of chemicals, climate change, pathogens, parasites and other environmental stressors in fish populations. For decades aquatic monitoring programs have included the collection of tissues preserved for microscopic pathology. Consequently, formalin-fixed, paraffin-embedded (FFPE) tissue can be an important reservoir of nucleic acids as technologies emerge that utilize molecular endpoints. Despite the cross-linking effects of formalin, its impact on nucleic acid quality and concentration, amplification, and sequencing are not well described. While fresh-frozen tissue is optimal for working with nucleic acids, FFPE [...]


Point of Contact :
Heather L Walsh
Originator :
Heather L Walsh, Vicki S Blazer, Adam J Sperry
Metadata Contact :
Heather L Walsh
Publisher :
U.S. Geological Survey
Distributor :
U.S. Geological Survey - ScienceBase
SDC Data Owner :
Eastern Ecological Science Center
USGS Mission Area :

Attached Files

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Supplementary Table 1.csv
“Normalized transcript abundance data”
40.71 KB text/csv


Data was collected to determine which fixative and fixation duration provided the least compromised nucleic acids which were extracted from laser microdissected liver of smallmouth bass.



  • Eastern Ecological Science Center
  • USGS Data Release Products



Additional Information


Type Scheme Key
DOI doi:10.5066/P9A12EL5

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