Astragalus species complex genetic data from southeast Utah (Grand County and San Juan County), USA
Data for journal manuscript: The demographic and ecological drivers of diversification among rare plant species
Dates
Publication Date
2021-04-08
Time Period
2019
Citation
Jones, M.R., Winkler, D.E., and Massatti, R., 2021, Astragalus species complex genetic data from southeast Utah (Grand County and San Juan County), USA: U.S. Geological Survey data release, https://doi.org/10.5066/P93SRC7M.
Summary
These data were compiled to investigate the evolutionary history of Astragalus iselyi, A. sabulosus var. sabulosus, and A. sabulosus var. vehiculus. The data release consists of six text files. One file is a bash script (astragalus_MLE_msms.sh) for generating simulated genetic data. Four files contain individual-level (astragalus.fasta, astragalus.nothin.recode.vcf, astragalus.recode.vcf) or population-level (astragalus_dadi.txt) information on genetic variation. One file contains sampling site-specific data for various soil and climatic variables (astragalus_env.txt). The .vcf files contain the full sequence information that is contained in the other files, but the file structures vary based on the programs used for analysis. These [...]
Summary
These data were compiled to investigate the evolutionary history of Astragalus iselyi, A. sabulosus var. sabulosus, and A. sabulosus var. vehiculus. The data release consists of six text files. One file is a bash script (astragalus_MLE_msms.sh) for generating simulated genetic data. Four files contain individual-level (astragalus.fasta, astragalus.nothin.recode.vcf, astragalus.recode.vcf) or population-level (astragalus_dadi.txt) information on genetic variation. One file contains sampling site-specific data for various soil and climatic variables (astragalus_env.txt). The .vcf files contain the full sequence information that is contained in the other files, but the file structures vary based on the programs used for analysis. These files may be opened and edited in a text editor program, such as Notepad ++ (PC) or BBEdit (Mac). The .vcf file can be loaded into the Stacks population program (Catchen et al. 2013) or VCFtools (Danecek et al. 2011) to calculate genetic diversity statistics (astragalus.nothin.recode.vcf contains multiple genetic variants per locus, whereas astragalus.recode.vcf contains only one genetic variant per locus).The astragalus_dadi.txt file can be used as an input file with the program dadi (Gutenkunst et al. 2009) to infer population history. Finally, astragalus.fasta can be loaded as an input file into RAxML (Stamatakis 2008) to perform phylogenetic analyses.
The purpose of these data is to understand the history of diversification and adaptation in an Astragalus species complex (Astragalus iselyi, A. sabulosus var. sabulosus, and A. sabulosus var. vehiculus) from eastern Utah. The objectives of collecting the data were to 1) understand population structure in this species complex, 2) estimate migration rates and divergence times between populations, 3) estimate effective population sizes, 4) and understand whether natural selection has operated on genetic variation within each population. The archived data can be further used to explore the evolutionary history of these taxa and the ecological variables that shape selection. This dataset can help in understanding the number of taxonomic units within this species complex and inform conservation strategies to effectively manage them.
Rights
The author(s) of these data request that data users contact them regarding intended use and to assist with understanding limitations and interpretation. Unless otherwise stated, all data, metadata and related materials are considered to satisfy the quality standards relative to the purpose for which the data were collected. Although these data and associated metadata have been reviewed for accuracy and completeness and approved for release by the U.S. Geological Survey (USGS), no warranty expressed or implied is made regarding the display or utility of the data for other purposes, nor on all computer systems, nor shall the act of distribution constitute any such warranty.
Preview Image
Astragalus sabulosus var. sabulosus from Utah (photo credit: Daniel Winkler)