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CALUX AhR human cell bioassays treated with water extracts from wells near Bemidji, MN USA (2018)

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Publication Date
Start Date
2019-02-15
End Date
2019-03-11

Citation

Bekins, B.A., Brennan, J.C., Tillitt, D.E., Cozzarelli, I.M., Illig, J.M., and Martinović-Weigelt, D., 2020, Dissolved organic carbon, total petroleum hydrocarbons and toxicity assay results for Bemidji, MN (2018)(ver. 2.0, June 2023): U.S. Geological Survey data release, https://doi.org/10.5066/P9EGYDRJ.

Summary

This data describes the relative luminescence units (RLUs) and relative fluorescence units (RFUs) of lysates of aryl-hydrocarbon responsive chemically-activated luciferase expression human hepatoma HG2L7.5c1 cells which were exposed for 4 or 24 h to beta-naphthoflavone dilutions, 2,3,7,8-tetrachlorodibenzo-p-dioxin dilutions, or dilutions of groundwater extracts collected from wells within or near an oil-contaminated area. This data also describes RFUs of bovine serum albumin standards which were used to convert the RFUs of the cell lysates to protein with which to normalize the RLUs of the cell lysates.

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Rel_ fluorescence_BSA_standards.csv 5.18 KB text/csv
Rel_luminescence_and_fluorescence_CALUX_cells.csv 99.42 KB text/csv

Purpose

The data were collected to obtain potencies of the water samples an aryl-hydrocarbon responsive cell line. Collaborators collected samples from the same sites are interested in PAH and PAH-metabolites in these water samples. Because our cell lines are responsive to these types of chemicals, the information from our data informs our collaborators which sites warrant further attention.

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