Soil microbes surrounding native and non-native Phragmites australis in the Great Lakes and East Coast of the United States (2015-2017 survey) (ver. 1.1, December 2020)
Dates
Publication Date
2020-10-22
Start Date
2016-08-10
End Date
2019-10-31
Revision
2020-12-28
Citation
Bickford, W.A., Zak, D.R., Kowalski, K.P., Snow, D.S., Dwortz, M.F., and Goldberg, D.E., 2020, Soil microbes surrounding native and non-native Phragmites australis in the Great Lakes and East Coast of the United States (2015-2017 survey) (ver. 1.1, December 2020): U.S. Geological Survey data release, https://doi.org/10.5066/P9HP8UXZ.
Summary
To determine the differences in soil microbial community composition between native and non-native lineages of Phragmites, we sampled soils from eight sites in the Great Lakes basin where populations of native and non-native Phragmites co-occurred. In addition, we included samples of soils from 27 populations of Phragmites across the Gulf of Mexico and Atlantic Coasts of the US. Samples were collected between July 2015 and September 2017. At each site in the Great Lakes, we sampled rhizosphere and bulk soil surrounding one ramet of each lineage. Samples from Atlantic and Gulf coasts were collected by homogenizing rhizosphere soils from multiple ramets of one population within a single lineage. DNA was extracted from soils and fungal, [...]
Summary
To determine the differences in soil microbial community composition between native and non-native lineages of Phragmites, we sampled soils from eight sites in the Great Lakes basin where populations of native and non-native Phragmites co-occurred. In addition, we included samples of soils from 27 populations of Phragmites across the Gulf of Mexico and Atlantic Coasts of the US. Samples were collected between July 2015 and September 2017. At each site in the Great Lakes, we sampled rhizosphere and bulk soil surrounding one ramet of each lineage. Samples from Atlantic and Gulf coasts were collected by homogenizing rhizosphere soils from multiple ramets of one population within a single lineage. DNA was extracted from soils and fungal, bacterial, and oomycete DNA was amplified to identify the microbial constituents. Amplicons were sequenced using Illumina MiSeq. This dataset includes outputs of bioinformatic analysis of sequences including operational taxonomic unit (OTU) generation, OTU abundance, resolved taxonomy, and environmental metadata collected in our survey. Raw sequences were uploaded to the NCBI Sequence Read Archive under SRA accession number PRJNA601975. First posted: October 22, 2020 (available by request) Minor Revision: December, 2020 (version 1.1)
Bickford, W.A., Zak, D.R., Kowalski, K.P., and Goldberg, D.E., 2020, Differences in rhizosphere microbial communities between native and non‐native
Phragmites australis
may depend on stand density: Ecology and Evolution, v. 10, no. 20, p. 11739–11751, https://doi.org/10.1002/ece3.6811.
Bickford, W.A., 2021, Data analysis and figures for Differences in Rhizosphere Microbial Communities Between Native and Non-Native Phragmites australis May Depend on Stand Density: U.S. Geological Survey software release, https://doi.org/10.5066/P93BBZWU
These data were collected to examine soil microbial community differences between the native and non-native lineages of Phragmites australis. Previous studies have found differences in rhizosphere bacterial communities in native and non-native Phragmites populations on the east and west coasts of the US. This data set explores whether similar relationships exist in populations of the Great Lakes Region and whether similar relationships exist in the fungal and oomycete communities of the rhizosphere.
Revision 1.1 by Wesley Bickford on December 28, 2020. To review the changes that were made, see ReleaseHistory_20201205.txt in the attached files section.
