Evaluation of ELISA for the Analysis of Imidacloprid in Plasma, Liver, and Fecal Matter

Dates

Publication Date: 2021-07-28
Start Date: 2016
End Date: 2020

Citation

Gross, M.S., Woodward, E.E.., and Hladik, M.L., 2021, Evaluation of ELISA for the Analysis of Imidacloprid in Plasma, Liver, and Fecal Matter: U.S. Geological Survey data release, https://doi.org/10.5066/P91WDVZ0.

Summary

Neonicotinoids have become the most widely used insecticides in world with rapid growth in applications as seed coatings. Nontarget organisms are exposed to concentrated levels of pesticidal active ingredients through ingestion of treated seeds. To better understand pesticide fate, analytical methods are necessary to rapidly screen and accurately quantitate contaminants in environmental and biological matrices. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is commonly employed for neonicotinoid analyses but requires expensive analytical instrumentation and potentially laborious sample preparation. Enzyme-linked immunosorbent assays (ELISAs) are efficient and sensitive alternative methods for neonicotinoid analyses, that [...]

Summary

Neonicotinoids have become the most widely used insecticides in world with rapid growth in applications as seed coatings. Nontarget organisms are exposed to concentrated levels of pesticidal active ingredients through ingestion of treated seeds. To better understand pesticide fate, analytical methods are necessary to rapidly screen and accurately quantitate contaminants in environmental and biological matrices. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is commonly employed for neonicotinoid analyses but requires expensive analytical instrumentation and potentially laborious sample preparation. Enzyme-linked immunosorbent assays (ELISAs) are efficient and sensitive alternative methods for neonicotinoid analyses, that does not require costly equipment. In this study, application of ELISA was compared to LC-MS/MS in the quantitation of imidacloprid in plasma, liver, and fecal matter from Japanese quail (Coturnix japonica) orally exposed to imidacloprid-treated wheat seeds. Two major imidacloprid metabolites, 5-OH-imidacloprid and imidacloprid-olefin, were found to cross-react within the immunoassay, confounding results. Imidacloprid concentrations from ELISA analysis ranged from 90.8-181 ng/mL, 7.90-40.4 ng/g, and 989-4404 ng/g in plasma, liver, and fecal matter, respectively. In comparison, LC-MS/MS imidacloprid concentrations ranged from 32.8-188 ng/mL for plasma, 18.7-116 ng/g for liver, and 51.3-427 ng/g for fecal matter. Imidacloprid concentrations determined by ELISA were generally higher in plasma and exceptionally higher in fecal matter compared to LC-MS/MS, Liver imidacloprid concentrations were higher when analyzed by LC-MS/MS. Good correlation was observed between imidacloprid concentrations determined in plasma (r2 = 0.93) and fecal matter (r2 = 0.89) by ELISA and LC-MS/MS, whereas a poor correlation (r2 = 0.35) was found in liver. In the analysis of these biological matrices, ELISA was found to be a good screening tool to measure active ingredients along with their metabolites. However, accurate quantitation should be completed with chromatographic based techniques such as LC-MS/MS.

Contacts

Point of Contact :: Michael S Gross
Process Contact :: Michael S Gross
Originator :: Michael S Gross, Emily E Woodward, Michelle L Hladik
Metadata Contact :: Michael S Gross
Distributor :: U.S. Geological Survey - ScienceBase
SDC Data Owner :: California Water Science Center
USGS Mission Area :: Ecosystems

Attached Files

sorting

Metadata First

Recent First

Alphabetical

Click on title to download individual files attached to this item.

Metadata for ELISA vs LCMSMS.xml Original FGDC Metadata	View	32.95 KB	application/fgdc+xml
00_Description of ELISA vs LCMSMS Imidacloprid Data.docx		23.47 KB	application/vnd.openxmlformats-officedocument.wordprocessingml.document
01_Cross-Reactivities_ImidaclopridELISA.csv		235 Bytes	text/csv
02_MatrixEffects_ImidaclopridELISA.csv		317 Bytes	text/csv
03_Plasma_ImidaclopridELISA.csv		1.31 KB	text/csv
04_Liver_ImidaclopridELISA.csv		1.34 KB	text/csv
05_FecalMatter_ImidaclopridELISA.csv		1.05 KB	text/csv
ELISA Plate Photo.jpg		196.19 KB	image/jpeg

Purpose

The collected data were used to compare enzyme-linked immunosorbent assay (ELISA) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis of imidacloprid in biological tissues and fecal matter from dosed Japanese quail (Coturnix japonica). Understanding the advantages and shortcomings of these analytical methods is essential in designing studies for either the screening or quantification of current-use pesticides in environmental and biological matrices.

Evaluation of ELISA for the Analysis of Imidacloprid in Plasma, Liver, and Fecal Matter

Dates

Citation

Summary

Summary

Contacts

Attached Files

sorting

Metadata First

Recent First

Alphabetical

Purpose

Preview Image

Map

Communities

Tags

Provenance

Additional Information

Identifiers

Item Actions

View Item as ...

Save Item as ...

View Item...

Dates

Citation

Summary

Summary

Contacts

Attached Files sorting Metadata First Recent First Alphabetical

Purpose

Preview Image

Map

Communities

Tags

Provenance

Additional Information

Identifiers

Item Actions

View Item as ...

Save Item as ...

View Item...

Attached Files

sorting

Metadata First

Recent First

Alphabetical