Data on the detection of Cumberlandia monodonta using a designed environmental DNA (eDNA) survey upstream and downstream of known populations in the Big Piney River, Missouri 2020 – 2022 (ver. 2.0, August 2024)
Dates
Publication Date
2023-08-07
Start Date
2019-09-24
End Date
2022-05-13
Revision
2024-08-21
Citation
Ruiz-Ramos, D.V., Thompson, N.L., Richter, C.A., and Klymus, K.E., 2023, Data on the detection of Cumberlandia monodonta using a designed environmental DNA (eDNA) survey upstream and downstream of known populations in the Big Piney River, Missouri 2020 – 2022 (ver. 2.0, August 2024): U.S. Geological Survey data release, https://doi.org/10.5066/P9923N3K.
Summary
Data describe a designed environmental DNA (eDNA) survey for the detection of Spectaclecase (Cumberlandia monodonta, also referred to as Margaritifera monodonta) from field collected water samples. Parameters described include the limit of detection and limit of quantification of the assay; a list of freshwater mussel species tissue samples that were used to test specificity of the assay; and field collected water samples that were tested to detect the presence of Spectaclecase DNA. Samples were collected from sites on the Big Piney River, Missouri from 2020 to 2022.
Summary
Data describe a designed environmental DNA (eDNA) survey for the detection of Spectaclecase (Cumberlandia monodonta, also referred to as Margaritifera monodonta) from field collected water samples. Parameters described include the limit of detection and limit of quantification of the assay; a list of freshwater mussel species tissue samples that were used to test specificity of the assay; and field collected water samples that were tested to detect the presence of Spectaclecase DNA. Samples were collected from sites on the Big Piney River, Missouri from 2020 to 2022.
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Detection_Cumberlandia_monodonta_eDNA_Metadata_20240821_Ver2.0.xml Original FGDC Metadata
View
54.83 KB
application/fgdc+xml
Cmon_eDNA_Quality_Assurance.csv
72.25 KB
text/csv
Cmon_eDNA_Samples.csv
208.1 KB
text/csv
Cmon_IPC_Quality_Assurance.csv
142.03 KB
text/csv
Cmon_LOD_LOQ.csv
8.59 KB
text/csv
Cmon_Sampling_Transect_ID.csv
879 Bytes
text/csv
Cmon_Specificity.csv
603 Bytes
text/csv
VersionHistory_Ver2.0.txt
1.6 KB
text/plain
Related External Resources
Type: ScienceBase Repository
Data on the detection of Epioblasma capsaeformis using a designed environmental DNA (eDNA) survey upstream and downstream of known populations in the Big Piney River, Missouri (2020 – 2022) and the Clinch River, Tennessee (2019 to 2021)
Environmental DNA survey data for Cumberlandia monodonta were collected to validate eDNA concentrations predicted by a one-dimensional hydraulic transport model that integrated high spatial and temporal resolution of system hydraulics with biological degradation of eDNA decay to simulate eDNA transport.