Skip to main content

Data on the detection of Cumberlandia monodonta using a designed environmental DNA (eDNA) survey upstream and downstream of known populations in the Big Piney River, Missouri 2020 – 2022 (ver. 2.0, August 2024)

Dates

Publication Date
Start Date
2019-09-24
End Date
2022-05-13
Revision
2024-08-21

Citation

Ruiz-Ramos, D.V., Thompson, N.L., Richter, C.A., and Klymus, K.E., 2023, Data on the detection of Cumberlandia monodonta using a designed environmental DNA (eDNA) survey upstream and downstream of known populations in the Big Piney River, Missouri 2020 – 2022 (ver. 2.0, August 2024): U.S. Geological Survey data release, https://doi.org/10.5066/P9923N3K.

Summary

Data describe a designed environmental DNA (eDNA) survey for the detection of Spectaclecase (Cumberlandia monodonta, also referred to as Margaritifera monodonta) from field collected water samples. Parameters described include the limit of detection and limit of quantification of the assay; a list of freshwater mussel species tissue samples that were used to test specificity of the assay; and field collected water samples that were tested to detect the presence of Spectaclecase DNA. Samples were collected from sites on the Big Piney River, Missouri from 2020 to 2022.

Contacts

Attached Files

Click on title to download individual files attached to this item.

Cmon_eDNA_Quality_Assurance.csv 72.25 KB text/csv
Cmon_eDNA_Samples.csv 208.1 KB text/csv
Cmon_IPC_Quality_Assurance.csv 142.03 KB text/csv
Cmon_LOD_LOQ.csv 8.59 KB text/csv
Cmon_Sampling_Transect_ID.csv 879 Bytes text/csv
Cmon_Specificity.csv 603 Bytes text/csv
VersionHistory_Ver2.0.txt 1.6 KB text/plain

Purpose

Environmental DNA survey data for Cumberlandia monodonta were collected to validate eDNA concentrations predicted by a one-dimensional hydraulic transport model that integrated high spatial and temporal resolution of system hydraulics with biological degradation of eDNA decay to simulate eDNA transport.

Map

Communities

  • Columbia Environmental Research Center (CERC)
  • USGS Data Release Products

Tags

Provenance

Revision 2.0 by Adrian Moore on August 21, 2022. To review the changes that were made, see “VersionHistory_Ver2.0.txt” in the attached files section.

Additional Information

Identifiers

Type Scheme Key
DOI https://www.sciencebase.gov/vocab/category/item/identifier doi:10.5066/P9923N3K

Item Actions

View Item as ...

Save Item as ...

View Item...