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Description of Work This spring (2014) we will measure Asian carp eDNA over time at a Missouri River site downstream of multiple spawning areas before and during spawning. We will measure the amount of Asian carp eDNA in water samples. The amount of eDNA will be related to the numbers of eggs and larvae counted in matched samples (water samples taken at the same time and place). The time since the eDNA was released by the carp will be estimated and these results will be related to the average age of AC eggs and larvae in matched samples. We will also test for substances which interfere with the eDNA measurement techniques and can lead to false negatives. We will also be validating markers developed by USGS and...
Carbon dioxide (CO2) is under consideration as a chemical fish deterrent at pinch points in rivers (e.g., inside locks) to reduce unwanted fish passage. This study evaluated the behavioral responses of invasive round goby (Neogobius melanostomus) to dissolved CO2 in laboratory tanks. The goal of this project was to quantify CO2 concentrations that modify round goby behavior. More specifically, avoidance behavior (voluntary response) and loss of equilibrium (involuntary response) were quantified across 5, 15, and 25°C water temperatures. These data can be used to inform future field testing of CO2 as an invasive fish deterrent. Spreadsheets include data that were used to evaluate the efficacy of carbon dioxide (CO2)...
There is growing interest in the use of carbon dioxide (CO2) as a management tool for controlling invasive fishes. However, there is limited published data on susceptibility of many commonly encountered species to elevated CO2 concentrations. Our objective was to estimate the 24-h LC50 and LC95 of four fishes (Rainbow Trout Oncorhynchus mykiss, Common Carp Cyprinus Carpio, Channel Catfish Ictalurus punctatus, and Westslope Cutthroat Trout Oncorhynchus clarkii lewisi). In the laboratory, we exposed fingerlings to a range of levels of CO2 for 24-h in unpressurized, flow-through tanks. A Bayesian hierarchical model was developed to estimate the dose response relationship for each species of fish with associated uncertainty,...
This is a dataset accompanying the 2017 publication “Assessing the efficacy of corn-based bait containing antimycin-a to control common carp populations using laboratory and pond experiments.” Data are included for the four experiments conducted described in this paper. First, data for a gavage experiment including mortality of carp per tank, fish metrics (lengths and weights), and water quality data (pH, dissolved oxygen, temperature). Second, data are included for the leaching trial including mortality of bluegill and carp, concentrations of antimycin in the tanks, fish metrics, and water quality metrics. Third, data are included for the species-specificity indoor trial. Included in this are mortality and sample...
This dataset includes information from ichthyoplankton sampling for silver carp and bighead carp collected from three main reaches in the Upper Mississippi River Basin, including the La Grange, Peoria, Starved Rock, and Marseilles reaches of the Illinois River, Pool 20 of Mississippi River, and the St. Croix River. Data include the number of bigheaded carp eggs and larvae identified using traditional manual sorting techniques, and genetic data derived from quantitative PCR (qPCR) and 4 bigheaded carp markers (SCTM4/5 and BHTM1/2) that were used to identify the amount of DNA in ichthyoplankton tow samples. These data provide information for natural resource agencies and managers who could use this qPCR screening...
Studied changes in the profiles of digestive enzymes of two planktivorous fishes, silver carp and gizzard shad. Results from this study will help scientists develop and design a novel oral delivery formulation to control the invasive silver carp while minimizing impacts on native fishes like the gizzard shad.
These datasets include data that was used to evaluate the efficacy of carbon dioxide (CO2) injected under-ice as a tool for reducing the overwinter survival of invasive carps.
This data contains environmental DNA (eDNA) sample assay results that were collected from water samples taken from a tank housing Asian carp and placed either on ice or at room temperature. At both treatment temperatures, water samples were left untreated or were treated with an ethanol and sodium acetate solution (EtOH-NaAc). This was done to evaluate an ethanol and sodium acetate solution to maintain the integrity of the DNA samples for the time between collection and lab testing. Every day for 6 days following collection, a subset of the samples was removed from each treatment and DNA was extracted and nuclear and mitochondrial markers were assayed with qPCR. Results showed comparable persistence of DNA between...
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Description of Work The field portion of a method validation study was completed August 2015. The study was designed to test the accuracy of a LAMP method to detect eDNA of Asian carps during simulated fish transport. During the study, more than 10,000 minnows were held in each of three fish transport tanks. Ten 4-inch silver carp were placed in one tank, a single 4-inch silver carp in the second tank while the third tank had no silver carp and served as a negative control. The LAMP method used amplifies the DNA of both bighead carp and silver carp. Three replicate trials were completed on separate days with side-by-side analyses completed by three novice and two expert users. Expert users had extensive experience...
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Data is a spreadsheet of the number of copies of Zebra Mussel DNA detected and the number of positive detections for Zebra Mussel DNA from water samples collected over 6 different substrates, at 4 depths in 2 lakes. The ash-free dry weight of the mussels at each of the sites is also included for each sampling location.
Bighead Carp, Silver Carp and Grass Carp (collectively Asian carp) were introduced to North America in the 1960s and 70s and have established in the lower Mississippi River basin. Previously published evidence for spawning of these species has been limited in the upper Mississippi River to just below Dam 22 (near Saverton, MO). In 2013 and 2014, we sampled ichthyoplankton at 18 locations in the main stem of the upper Mississippi River from Dam 9 through Dam 19 and in four tributary rivers that empty into the Mississippi River (Des Moines, Skunk, Iowa and Wisconsin Rivers). We identified eggs and larvae using morphological techniques and then used genetic tools to confirm species identity. The spawning events we...
This was a joint project between: Upper Midwest Environmental Sciences Center, U.S. Geological Survey, La Crosse, WI 54603 La Crosse Fish Health Center, U.S. Fish and Wildlife Service, Onalaska, WI 54650 This repository contains the raw data and R codes used in the metabolomics analysis for the manuscript which used silver and bighead carp fry cells exposed to thiram. Species indclude in this manuscript Silver Carp (Hypophthalmichthys molitrix) Bighead Carp (Hypophthalmichthys nobilis) The raw data is in: BHC thiram data.csv SVC thiram data labels.csv The code used in the ordination analysis is in: figure_1_knit_code.Rmd The code used in the principle component analysis is in: figure_2_knit_code.Rmd The...
Categories: Data
Spreadsheets include data that was used to evaluate the efficacy of carbon dioxide (CO2) deterrence at different temperatures in invasive carps. There are 8 csv files and 2 folders containing text documents associated with this study.
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Environmental DNA results for water samples collected at USGS streamgages in the Columbia and Colorado river basins. Water samples were collected by USGS hydrologic technicians May - September 2018 and analyzed for presence of kokanee and yellow perch DNA. In this dataset, we report results and applicable covariates associated with each streamgage location. This is version 1.1 of this data release. The Kokanee_Perch_eDNA data and metadata files have been revised to correct for improper attribution of 16 samples. For access to previous versions, please contact the Point of Contact identified on this page.
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Environmental DNA results for water samples collected at USGS streamgages in the Columbia and Colorado river basins. Water samples were collected by USGS hydrologic technicians May - September 2018 and analyzed for presence of dreissenid mussel DNA. In this dataset, we report quantitative PCR results for each streamgage location.
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Environmental DNA quantitative PCR results for water samples collected in 2018 at USGS streamgages downstream of Columbia River Basin reservoirs that are considered to have moderate to high suitability for invasive dreissenid mussels. Water samples were tested for DNA of dreissenid mussels, kokanee salmon, and yellow perch. This is version 1.1 of this data release. The Kokanee_Perch_eDNA data and metadata files have been revised to correct for improper attribution of 16 samples. For access to previous versions, please contact the Point of Contact identified on this page. First Posted March 29, 2019 Revised July 19, 2019, ver 1.1
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Description of Work Studies assessing seasonal and spatial changes in digestive enzymes and gill raker morphology in bigheaded carps and native planktivorous fishes have been completed. Results indicate that bigheaded carps feed earlier in the year than native filter-feeding fishes and that certain digestive enzymes present in bigheaded carps are either not present in some native fishes or are much less active in the native species than in bigheaded carps. Results also indicate that the gill raker morphology of bigheaded carps is relatively constant with minimal seasonally or spatially relevant changes unlike that of the native gizzard shad (Dorosoma cepedianum) which had both seasonal and spatially correlated changes...
Resource agencies are searching for effective methods to prevent the spread of invasive Silver Carp (Hypophthalmichthys molitrix) and Bighead Carp (Hypophthalmichthys nobilis), hereafter bigheaded carps, from the Mississippi River basin into the Laurentian Great Lakes. Elevating carbon dioxide (CO2) concentrations in water within locks and other pinch points is an approach being considered to reduce invasive fish passage. Laboratory studies have shown that bigheaded carps strongly avoid areas of elevated CO2 (Kates et al. 2012; Dennis et al. 2015). Similarly, telemetry studies found that CO2 can be used to exclude bigheaded carps from certain locations (Donaldson et al. 2016) and reduce upstream movement (Cupp et...
Spreadsheets include data that was used to evaluate the efficacy of dry ice administered under-ice as a tool for reducing the overwinter survival of invasive carps. There are 10 CSV associated with this study
    map background search result map search result map Environmental DNA mapping of Zebra Mussel populations: Data Environmental DNA surveillance data for USGS streamgage sampling in the Columbia River Basin, 2018 Dreissenid mussel eDNA results for streamgage water samples in the Columbia and Colorado River basin Fish eDNA results for streamgage water samples in the Columbia and Colorado River basins Environmental DNA mapping of Zebra Mussel populations: Data Environmental DNA surveillance data for USGS streamgage sampling in the Columbia River Basin, 2018 Dreissenid mussel eDNA results for streamgage water samples in the Columbia and Colorado River basin Fish eDNA results for streamgage water samples in the Columbia and Colorado River basins