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This dataset consists of measurements of the digestive gland in the freshwater snail Lymnaea stagnalis orally exposed to uranium. The objective of the study was to assess the morphological effects of uranium on the snail digestive gland by light microscopy. Laboratory-cultured L stagnalis were exposed to either synthetic or natural solid phases of uranium and a subset (6 controls and 6 exposed) were euthanized and fixed in 10% neutral buffered formalin for histopathological assessment. Slides were processed and stained with hematoxalin and eosin as per standard procedure (Luna 1968). The size of each sample, the length and width of digestive gland tubules (5 random glands per sample) and height of digestive cells...
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This dataset contains field measurements of lakebed groundwater temperatures collected during three surveys in July 2008, August 2014, and August 2015, by using a handheld thermocouple probe and GPS at a permeable reactive barrier at Ashumet Pond, Falmouth, MA. The comma-separated file includes unique site identifiers, locations of measurement points, temperatures, dates, and types of measurement (groundwater, surface water, or control). Also included are ESRI raster datasets for each measurement date for (1) interpolated lakebed groundwater temperatures, and (2) interpolated lakebed groundwater temperatures normalized to the surface water temperature at the time of measurement. This data release is provided...
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Data from study assessing the utility of knowledgebase-leveraging of comprehensive environmental-contaminant-exposure datasets by comparing biological effects predicted on the basis of target chemical analyses with measured biological effects in corresponding split water samples.
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Coastal communities are uniquely vulnerable to sea-level rise (SLR) and severe storms such as hurricanes. These events enhance the dispersion and concentration of natural and anthropogenic chemicals and pathogenic microorganisms that could adversely affect the health and resilience of coastal communities and ecosystems in coming years. The U.S. Geo­logical Survey has developed the Sediment-Bound Contaminant Resiliency and Response (SCoRR) strategy to define baseline and post-event sediment-bound environmental health (EH) stressors. These data document toxicity measured by reduction of the light emission of Aliivibrio (formerly Photobacterium) fischeri and the inhibition of polymerase chain reactions caused by environmental...
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Reproductive Data for All Nests Monitored; Data for Mayfield Analysis; Eggshell thickness, nestling morphological measurements, nestling plasma stable isotopes, and nestling red blood cell oxidative DNA damage; Egg organochlorine pesticide, total PCB, flame retardants, and PCB toxic equivalent concentrations; Fish capture by osprey; Fish pools and size for contaminant analysis; Fish organochlorine pesticide, total PCB and flame retardants concentrations; Data for estimation of p,p’-DDE and total PCB biomagnification from fish to osprey eggs
These data are comprised of measurements of gross alpha activity, gross beta activity, and radionuclide activities (isotopic U, isotopic Th, Ra-226) in small rodent bodies. Samples were collected at non-mining and U breccia pipe mining sites in various production stages, in the Grand Canyon watershed.
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Over 300 samples were collected in New York, Vermont, and Massachusetts from wastewater treatment facilities (20 locations), drinking water facilities (9 locations), streams (53 locations), lakes (2 locations), a bay (1 location), and a POCIS (Polar Organic Chemical Integrative Sampler) blank sample between April 2009 and October 2018. This data release provides chemistry data for over 400 analytes, including pharmaceuticals, hormones, personal care and domestic use compounds, pesticides and other compounds using 10 different laboratory analytical methods on water, sediment, wastewater sludge, and POCIS samples. Quality assurance data, such as equipment and field blank data and percent recovery for isotopic dilution...
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These data were compiled to study mercury and selenium concentrations in fish species and assemblages in lotic waterbodies across the Upper Colorado River Basin. Data were compiled from State and Federal agencies. This data table contains raw concentration data, as well as standardized concentrations corrected for differences based on sample type (i.e., tissue type), species-specific bioaccumulation rates (Table S1), and fish size (Table S2). The data were used in linear mixed effects models to estimate average mercury and selenium concentration in fish species and in fish assemblages, including fish total length (cm), sampling location (Sub basin name and GPS coordinates), and sampling year (Figures 2,3, and 4...
This work is part of a study of the immunological effects of exposure to alternative flame retardants in avian species. For the pathology portion of the study, hatchling American kestrels (Falco sparverius) were exposed to the flame retardant isopropyl triphenyl phosphate (ITP) and then challenged with a synthetic analogue of viral double-stranded RNA, polyinosinic:polycytidylic acid (poly I:C). Control birds were challenged with vehicle only or vehicle and poly I:C. At euthanasia, spleen, thymus, and bursa of Fabricius were collected and fixed in 10% neutral buffered formalin for histopathological assessment. Slides were processed and stained with hematoxalin and eosin as per standard procedure (Luna 1968). Quantitative...
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This dataset contains field measurements of temperature in shallow vertical profiles beneath a lake-bottom in the vicinity of a permeable reactive barrier (PRB) at Ashumet Pond, Falmouth, MA. From August 21, 2015 – September 3, 2015 vertical temperature profilers designed to quantify groundwater upwelling dynamics were installed at seven locations. Hollow stainless steel pipes (2.5 cm OD) were slotted horizontally to allow installation of iButton thermal data loggers (Maxim Integrated DS1922L, 0.0625 oC precision) protected with waterproof silicone caulking. The temperature profilers were gently tapped into the cobbly bed by first driving guide-hole of slightly smaller diameter. After installation, the thermal loggers...
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This dataset contains the trace element concentration results for samples collected from tapwater sites in East Chicago, Indiana, August-September, 2017, and Chicago, Illinois, July-December, 2017. Samples were collected one time, from 45 private residences and associated drinking water plants and source water locations. Water-quality samples were analyzed at two U.S. Geological Survey laboratories: National Water Quality Laboratory, Denver, Colorado and at the Redox Chemistry Laboratory, Boulder, Colorado.
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A seminal question in ecotoxicology is the extent to which contaminant exposure evokes prolonged effects on physiological function and fitness. A series of studies were undertaken with American kestrels ingesting environmentally realistic concentrations of the second-generation anticoagulant rodenticide (SGAR) brodifacoum (BROD). Kestrels fed BROD at 0.3, 1.0 or 3.0 µg/g diet wet wt for 7 d exhibited dose-dependent hemorrhage, histopathological lesions and coagulopathy (prolonged prothrombin and Russell’s viper venom times). Following termination of a 7 d exposure to 0.5 µg BROD/g diet, prolonged blood clotting time returned to baseline values within a week, but BROD residues in liver and kidney (terminal half-life...
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A heterologous thrombin clotting time assay (TCT) was used to measure the time for conversion of fibrinogen to fibrin using commercially available reagents. Human reference material included in the kit was diluted with imidazole buffered saline (IBS; 0.0125M imidazole-0.109 M sodium chloride, pH7.4) to generate a standard curve. Each kestrel plasma sample was thawed at 37ºC and diluted with IBS, and following incubation at 37ºC, the reaction was then initiated by the addition of bovine thrombin reagent supplied with the assay kit, with clotting time measured to 0.1 s. Fibrinogen concentration was determined in a single assay for each of the 3 study trials, with reference samples interspersed among study samples....
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Range finding trial in which kestrels were fed diets containing varying quantities of brodifacoum and signs of intoxication were monitored.
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This dataset consists of concentration and isotopic data for a method designed to isolate and analyze methylmercury. Several matrices (lab-based, and natural) were tested to ensure that the method was capable of recovering methylmercury amendments. Additionally, natural matrices (plankton and sediment) with ambient methylmercury were also analyzed to ensure that the method could be successfully applied to natural matrices. Separation tests for sediment, water, and biotic matrices showed acceptable recoveries (98 ± 5%, n = 54) and reproducible δ202Hg isotope results (2SD < 0.15 ‰) down to 5 ng of MeHg. This dataset is part of a complimentary paper published in Analytical and Bioanalytical Chemistry (https://doi.org/10.1007/s00216-019-02277-0)...
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These data are comprised of measurements of aluminum, iron, cobalt, nickel, copper, zinc, arsenic, selenium, molybdenum, silver, cadmium, thallium, lead, bismuth, thorium, uranium, and mercury in invertebrates, vegetation, and small rodents. Gross alpha activity, gross beta activity, and radionuclide activities (isotopic U, isotopic Th) are also presented for vegetation; radioactivities for small rodents were previously presented at https://doi.org/10.5066/P9HARTQ4. Histopathology results are given for liver and kidney lesions generally associated with metals toxicoses in small rodents. Samples were collected at the Pinenut Mine (active mining) and Arizona 1 Mine (post-production) in the Grand Canyon watershed....
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This is a collection of data on the contamination of water, fish, and sediments in Biscayne National Park and in canals that discharge into the national park. The water data are estimations of their concentrations in water based on sampling with passive water samplers (semi-permeable membrane devices and polar organic chemical integrative samplers). Also in this data set are estrogen equivalencies for the chemical mixtures in the polar organic chemical integrative samplers.
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This dataset contains results for samples collected at stormwater basins across the United States to assess the potential contaminant pathways of these basins to surface water and groundwater. Sample sites were in stormwater conveyance infrastructure that discharged mixed stormwater runoff from buildings, parking lots, roads, and other infrastructure in residential, commercial, and industrial landscapes prior to surface-water discharge or groundwater infiltration. Stormwater basins draining various types of urban environments, and of various drainage areas, were selected and sampled during storm events. The associated report (Masoner and others, 2019) can be found at https://doi.org/10.1021/acs.est.9b02867. A total...
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This dataset contains field measurements of direct groundwater seepage by using half-barrel style seepage meters permanently installed in a lake-bottom permeable reactive barrier (PRB) at Ashumet Pond, Falmouth, MA. The meters are a modified version of a standard half-barrel seepage meter. Each meter consists of a 208-L polyethylene, corrosion-inhibiting drum cut to a length of 0.8 m and fitted with a removable 0.56-m-diameter lid. A 1.3-cm-diameter hole was drilled into each lid to allow outflow or inflow to be measured. During installation, the top rim of each barrel was positioned to be at the expected grade after backfilling. Between 2004 and 2015, seepage rates at each meter were measured annually, with the...
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This data release includes comma-delimited datafiles (.CSV files) and geospatial datasets (GIS shapefile and raster datasets) from a survey of lake-bottom temperatures using a Fiber-Optic Distributed Temperature Sensing (FO-DTS) system in May 2007. The distribution of lakebed temperatures was determined over an 18-hour period in May 2007 using a LIOS fiber-optic distributed temperature sensing (FO-DTS) system (LIOS Technology, Cologne, Germany) connected to a 1000-m-long fiber cable shielded with rubberized armoring. The FO-DTS system measures temperature by means of optical fibers that function as linear sensors of temperature dependent laser light backscatter, producing temperature values to an accuracy of 0.01oC...
map background search result map search result map Baseline aquatic contamination and endocrine status in a resident fish of Biscayne National Park Matrix inhibition PCR and Microtox® 81.9% screening assay analytical results for samples collected for the Sediment-Bound Contaminant Resiliency and Response Strategy pilot study, northeastern United States, 2015 Fish tissue mercury and selenium concentrations in Upper Colorado River Basin: 1962-2011 Occurrence and Concentrations of Trace Elements in Discrete Tapwater Samples Collected in Chicago, Illinois and East Chicago, Indiana, 2017 01_Field measurements of lake-bottom groundwater temperatures from fiber-optic distributed temperature sensing (FO-DTS), Ashumet Pond, May 30-31, 2007 Examination of contaminant exposure and reproduction of ospreys (Pandion haliaetus) nesting in Delaware Bay and River in 2015 02_Field measurements of lake-bottom seepage near a permeable reactive barrier, Ashumet Pond, Falmouth, MA 03_Field measurements of temperature in vertical lake-bottom profiles near a permeable reactive barrier, Ashumet Pond, Falmouth, MA 04_Field measurements of lakebed groundwater temperatures by using a thermocouple probe, July 2008, August 2014, and August 2015, Falmouth, MA Chemical-Gene and Chemical-Pathway Interactions Predicted for Chemicals Detected in the USGS-USEPA National Streams Pilot Study Based on Effects Data in the Comparative Toxicogenomics Database (CTD) Concentrations of inorganic and organic analytes in a national reconnaissance of urban stormwater runoff to surface water and groundwater in the United States Brodifacoum toxicity in American kestrels (Falco sparverius) with evidence of increased hazard upon subsequent anticoagulant rodenticide exposure 1. Results of Trial 1 4. Results of Blood Clotting Assays Isolation of Methylmercury Using Distillation and Anion-Exchange Chromatography for Isotopic Analyses in Natural Matrices Data Release Chemical analyses and histopathology of organisms and plants collected from breccia pipe uranium mine sites in the Grand Canyon watershed, 2015-2020 Emerging contaminant data at wastewater treatment facilities, municipal water supplies, surface water and lake sites in the northeastern United States, 2009-2018 (ver. 1.1, January 2023) 01_Field measurements of lake-bottom groundwater temperatures from fiber-optic distributed temperature sensing (FO-DTS), Ashumet Pond, May 30-31, 2007 02_Field measurements of lake-bottom seepage near a permeable reactive barrier, Ashumet Pond, Falmouth, MA 03_Field measurements of temperature in vertical lake-bottom profiles near a permeable reactive barrier, Ashumet Pond, Falmouth, MA Brodifacoum toxicity in American kestrels (Falco sparverius) with evidence of increased hazard upon subsequent anticoagulant rodenticide exposure 1. Results of Trial 1 4. Results of Blood Clotting Assays Baseline aquatic contamination and endocrine status in a resident fish of Biscayne National Park Occurrence and Concentrations of Trace Elements in Discrete Tapwater Samples Collected in Chicago, Illinois and East Chicago, Indiana, 2017 Chemical analyses and histopathology of organisms and plants collected from breccia pipe uranium mine sites in the Grand Canyon watershed, 2015-2020 Examination of contaminant exposure and reproduction of ospreys (Pandion haliaetus) nesting in Delaware Bay and River in 2015 Isolation of Methylmercury Using Distillation and Anion-Exchange Chromatography for Isotopic Analyses in Natural Matrices Data Release Fish tissue mercury and selenium concentrations in Upper Colorado River Basin: 1962-2011 Emerging contaminant data at wastewater treatment facilities, municipal water supplies, surface water and lake sites in the northeastern United States, 2009-2018 (ver. 1.1, January 2023) Matrix inhibition PCR and Microtox® 81.9% screening assay analytical results for samples collected for the Sediment-Bound Contaminant Resiliency and Response Strategy pilot study, northeastern United States, 2015 Concentrations of inorganic and organic analytes in a national reconnaissance of urban stormwater runoff to surface water and groundwater in the United States Chemical-Gene and Chemical-Pathway Interactions Predicted for Chemicals Detected in the USGS-USEPA National Streams Pilot Study Based on Effects Data in the Comparative Toxicogenomics Database (CTD)