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This Data Release makes available measurements of phytoplankton species composition, abundance and cell size made on samples collected in San Francisco Bay (CA) from April 1992 through March 2014. Phytoplankton samples were collected at 31 stations along a 145-km transect where the variability of salinity, temperature, turbidity and nutrient concentrations reflected a broad range of environmental factors that regulate phytoplankton growth and abundance (map of sampling locations is available on the project website at: https://sfbay.wr.usgs.gov/access/wqdata/overview/wherewhen/stnmap.html). Whole water samples were preserved with acid Lugol’s solution, and 2 to 50 ml aliquots were settled in chambers for 6 to 24...