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Interest in the field of environmental DNA (eDNA) is growing rapidly and eDNA surveys are becoming an important consideration for aquatic resource managers dealing with invasive species. However, in order for eDNA monitoring to mature as a research and management tool, there are several critical knowledge gaps that must be filled. One such gap is the fate of eDNA materials in the aquatic environment. Understanding the environmental factors that influence the decay of eDNA and how these factors impact detection probabilities over time and space could have significant implications for eDNA survey design and data interpretation. Here we experimentally explore eDNA decay in waste materials and reproductive cells obtained...
Categories: Data;
Types: Citation;
Tags: Aquatic Biology,
Ecology,
Environmental Health,
Genetics,
Missouri,
These data are comprised of measurements of gross alpha activity, gross beta activity, and radionuclide activities (isotopic U, isotopic Th, Ra-226) in small rodent bodies. Samples were collected at non-mining and U breccia pipe mining sites in various production stages, in the Grand Canyon watershed.
Data collected from a comprehensive set of hydrodynamic experiments conducted with live grass carp eggs and larvae, to evaluate drifting and swimming patterns with 3 different in-stream obstructions: (1) a gravel bump, (2) a single cylinder, and (3) submerged vegetation. The hydrodynamic behavior of eggs and larvae with each obstruction was continuously monitored for about 85 consecutive hours. Transient spatial distributions of the locations of eggs and larvae across the water column were generated for each flow scenario.
Data from metabarcoding assays to detect a suite of mussel species using mitochondrial DNA regions of the cytochrome c oxidase subunit I (COI) and NADH dehydrogenase subunit (ND1) genes sequences.
Data describe a designed environmental DNA (eDNA) survey for the detection of longnose darter (Percina nasuta) in field collected water samples. Parameters described include the limit of detection and limit of quantification of the assay; a list of freshwater fish species tissue samples that were used to test specificity of the assay; and field collected water samples that were tested to detect the presence of longnose darter DNA. Samples were collected from sites on the St. Francis river, Missouri in 2018.
Data consist of genotypes for pallid sturgeon and paddlefish broodstock and artificially-produced larvae at 19 sturgeon microsatellite loci and four paddlefish loci. Microsatellite genotypes allowed confirmation of homozygous sturgeon DNA and absence of paddlefish DNA in haploid gynogens. Data also consist of DNA yield estimates for haploid gynogens for proof of concept that haploid gynogens can provide sufficient DNA quantity for restriction site-associated DNA sequencing studies.
Text files containing data regarding attributes of lake trout egg thiamine concentrations from sites in lakes Huron and Michigan collected during 2019-20, sampling site locations and quality assurance quality control values for comparison to previous years (2017-18) lake trout egg thiamine concentrations.
Diploid and triploid Grass Carp (Ctenopharyngodon Idella) tagged with acoustic transmitters were observed in Truman Reservoir, Missouri from February 2017 to March 2019 to determine habitat usage in winter. Habitat parameters were measured at used and available sites to model habitat preference. In addition, tagged fish were targeted during removal attempts to determine the efficacy of using tagged Judas Grass Carp in removing wild conspecifics. This dataset includes information on tagged Grass Carp and habitats they selected as well as capture events and characteristics of the wild fish caught during these attempts.
Vegetative buffer strips have been demonstrated to effectively reduce loads of sediment, nutrients, and herbicides in surface runoff, but their effectiveness for reducing veterinary antibiotic loads in runoff has not been well documented. Surface runoff simulation where performed to determine the effectiveness of Vegetative buffer strips vegetation and width on surface runoff loads of the veterinary antibiotics sulfamethazine and lincomycin. The three datasets in this release document 1. physical and chemical soil properties for soil located on experimental vegetated buffer strip plots prior to surface runoff simulations; 2. Rainfall simulator performance data and antecedent soil water content data for all vegetated...
The health of soils along roadways is critical for maximizing habitat quality and minimizing negative ecological effects of roads. Adjacent to unpaved roads, soil chemistry may be altered by the deposition of dust, as well as by road treatment with dust suppressants or soil stabilizer products. If present in roadside soils, these product residues may be available to plants, terrestrial invertebrates, or small mammals. Unfortunately, very few studies have attempted to track the transport of dust suppressants after application. As part of a larger ongoing study on the environmental effects of dust suppressant products on roadside plants and animals, we sampled roadside soils at Loess Bluff National Wildlife Refuge...
Categories: Data;
Tags: Road ecology,
USGS Science Data Catalog (SDC),
Unpaved road,
dust,
dust suppressant
Data represent reports of capture of black carp by commercial fishers and biologists with information regarding size characteristics of collected individuals, dimensions of capture gears, and spatial and temporal distributions of captures.
Survival, behavior, reproduction, morphometric measurements, and tissue analyses of crayfish, mussels, and fish from acute toxicity tests with pyrethroid pesticides. Exposure and recovery water quality data are also included for quality assurance purposes.
This dataset contains the results of a laboratory chronic bioassay with 6-week-old juvenile mussels (Fatmucket, Lampsilis siliquoidea) in a 28-day zinc exposure (120 and 240 micrograms zinc per liter) with endpoints of survival and growth (biomass and length) following standard ASTM methods. Surviving mussels were transferred from the control and treatment groups into a culture pond and their survival and growth were monitored for 56 days. In this data release we report individual biological, chemical, and water quality measurements from the laboratory toxicity test exposure and pond grow-out period.
Data were collected in association with the application of two physical barrier treatments, bentonite clay (a sealing agent) and expanding foam (an insulation sealant), in natural pond-burrows to suppress or kill the invasive red swamp crayfish (Procambarus clarkii). Data include burrow diameter and depth, crayfish sex and size, and determination of the treatment reaching the burrow chamber.
This dataset provides results for the following: (1) metals concentrations in soils surrounding breeding song bird nest sites; (2) metals concentrations in invertebrates (earthworm and grub composites) collected near nest sites; (3) metals concentrations and delta-aminolevulinic acid dehydratase (ALAD) activity in avian blood samples; and (4) metals concentrations, indicators of oxidative stress and DNA damage, and histopathology in liver and/or kidney tissues collected from breeding song birds.
This dataset documents locations of and water temperature recorded by Hobo Water Temp Pro series loggers (Onset Computer Corp., Bourne, MA) deployed by the USGS Comprehensive Sturgeon Research Project at up to 24 locations in the Lower Missouri River (from the Gavins Point Dam near Yankton, South Dakota to its confluence with the Mississippi River near St. Louis, Missouri) and 13 selected tributaries (James River, Big Sioux River, Little Sioux River, Platte River (in Nebraska), Nishnabotna River, Nodaway River, Platte River (in Missouri), Kansas River, Grand River, Chariton River, Lamine River, Osage River, and Gasconade River) since 2005. Data document the site name, spatial location, temporal extent of water temperature...
The vernal pool fairy shrimp, Branchinecta lynchi, (Branchiopoda; Anostraca) and other fairy shrimp species have been listed as ‘threatened’ or ‘endangered’ under the United States’ Endangered Species Act (ESA). There is little information about the sensitivity of Branchinecta spp. to toxic effects of contaminants, making it difficult to determine whether they are adequately protected by water quality criteria. A series of acute (24 hr) lethality/immobilization tests were conducted with three species of fairy shrimp (Branchinecta lynchi, Branchinecta lindahli, and Thamnocephalus platyurus) and 10 chemicals with varying modes of toxic action: ammonia, potassium, chloride, sulfate, chromium(VI), copper, nickel, zinc,...
Categories: Data;
Types: Citation;
Tags: NA,
USGS Science Data Catalog (SDC),
acute toxicity,
vernal pool fairy shrimp,
water-only toxicity
This dataset characterizes sensitivity of fathead minnow and a unionid mussel to elevated major ions in produced water from oil and gas extraction process.
Survival and growth of four species of juvenile mussels in a pulsed flow-through auto-feeding beaker system.
Categories: Data;
Tags: Columbia Environmental Research Center,
Freshwater mussels,
Growth,
Survival,
USGS Science Data Catalog (SDC),
These data are comprised of measurements of aluminum, iron, cobalt, nickel, copper, zinc, arsenic, selenium, molybdenum, silver, cadmium, thallium, lead, bismuth, thorium, uranium, and mercury in invertebrates, vegetation, and small rodents. Gross alpha activity, gross beta activity, and radionuclide activities (isotopic U, isotopic Th) are also presented for vegetation; radioactivities for small rodents were previously presented at https://doi.org/10.5066/P9HARTQ4. Histopathology results are given for liver and kidney lesions generally associated with metals toxicoses in small rodents. Samples were collected at the Pinenut Mine (active mining) and Arizona 1 Mine (post-production) in the Grand Canyon watershed....
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