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A large spill of wastewater from oil and gas operations was discovered adjacent to Blacktail Creek near Williston, North Dakota in January 2015. To determine the effects of this spill on streambed microbial communities over time, bed sediment samples were taken from Blacktail Creek upstream, adjacent to, and at several locations downstream from the spill site. Blacktail Creek is a tributary of the Little Muddy River, and additional samples were taken upstream and downstream from the confluence of Blacktail Creek and the Little Muddy River. Samples were collected in February 2015, June 2015, June 2016, and June 2017. DNA was extracted from these sediments, and sequencing of the 16S ribosomal RNA gene was performed...
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The dataset includes bacteria concentrations and microbial source indicator concentrations collected by Daniel B. Stephens and Associates, Inc. on behalf of the City of Albuquerque in the Middle Rio Grande in Albuquerque, NM, in 2018.
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Data were collected to evaluate the efficacy of quantitative polymerase chain reaction (qPCR) as a rapid, alternative method for monitoring recreational water at select beaches and rivers of Sleeping Bear Dunes National Lakeshore (SLBE), in Empire, Michigan. Water samples were collected between August 4 and September 18, 2014 (N=297) from four locations (Esch Rd, Otter Creek, Platte Bay, and Platte River). The samples were analyzed for indicator bacteria, E. coli and enterococci, by both culture-based (membrane filtration, MF; traditional method) and non-culture based (qPCR; rapid method). Recreational water quality standards (RWQS) and beach action values (BAV) were used as indices to compare water quality standard...
At the Naturita site in Colorado, USA, groundwaters were sampled and analyzed for chemical composition and by culture and culture-independent microbiological techniques. In addition, sediments were extracted with a dilute sodium carbonate solution to determine quantities of labile uranium within the sediments. Samples from the upgradient portion of the contaminated aquifer, where very little dissolved Fe(II) is found in the groundwater, have uranium content that is controlled by U(VI) adsorption and few metal-reducing bacteria are observed. In the extreme downgradient portion of the aquifer, where dissolved Fe(II) is observed, uranium content of the sediments includes significant quantities of reduced U(IV) and...
We evaluated and optimized three rapid methods for extraction of high-quality DNA from carbonate-encrusted microbial communities using modern calcifying oncolites built by cyanobacteria and diatoms in a high-calcium freshwater river. Pulverization, acid (HCl) dissolution, and chelator-mediated (EDTA) dissolution of the carbonate matrix were used and optimized to liberate microbial cells from their mineral encasing. This was followed by cell lysis and DNA extraction and isolation. HCl dissolution yielded no measurable DNA, probably due to hydrolysis, whereas pulverization and EDTA dissolution yielded averages of 3.5 and 7.8 μg per gram of sample, respectively, of high molecular weight DNA. These DNA isolates...
Fatty acid (FA) analysis is a promising tool to study trophic relationships in soil food webs. We determined FA biomarkers to trace bacterial food sources (Bacillus megaterium, Pseudomonas putida, Enterobacter aerogenes) of Collembola (Heteromurus nitidus, Protaphorura fimata, Folsomia candida). In addition, ?15N, ?13C, C/N ratio, body weight and NLFA/PLFA ratio (neutral lipid/phospholipid fatty acids) of Collembola were assessed. These measures indicated that P. putida ranked first, B. megaterium second and E. aerogenes third in food quality. FAs specific for bacteria were found in the NLFAs of the Collembola reflecting the respective bacterial diet. Biomarker FAs for gram-positive bacteria were methyl branched...
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These datasets are inputs to LOADEST regression models for estimating E. coli concentration in the Missouri River at four sites near Omaha, Nebraska. The datasets described by this metadata are daily data and include precipitation data and Long Term Control Plan (LTCP) progress (projects completed and combined sewer overflows [CSO] upstream of the sampling site) data. These data sets are in .csv format. Some model inputs are not included in this data release because they are published elsewhere (National Water Information System- web interface https://waterdata.usgs.gov/nwis). The file Daily_and API_Precip_Variables.csv contains daily precipitation from January 1, 2012 through October 1, 2016 for the National Weather...
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This data release contains eight datasets and metadata related to streamwater constituent load estimation and E. coli bacteria concentration predictions at 15 watersheds in DeKalb County, Georgia for 2012 to 2016 (the water-quality model calibration data goes through 9/22/2017 and the water-quality assurance samples goes through 11/7/2017). Loads were estimated for 15 constituents: biochemical oxygen demand, chemical oxygen demand, total suspended solids, suspended sediment concentration, total nitrogen, total nitrate plus nitrite, total phosphorus, dissolved phosphorus, total organic carbon, total calcium, total magnesium, total copper, total lead, total zinc, and total dissolved solids. The data release includes...
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Bacteria were isolated from seeds of non-native Phragmites australis (haplotype M) then representatives were evaluated for their capacities to become intracellular in root cells, and their effects on: 1.) germination rates and seedling growth, 2.) susceptibility to damping-off disease, and 3.) mortality and growth of competitor plant seedlings (dandelion (Taraxacum officionale F. H. Wigg) and curly dock (Rumex crispus L.)). The experiments included the following assessments of Phragmites-associated bacteria (Pseudomonas spp, strains Sandy LB4 (Pseudomonas fluorescens) and West 9 (Pseudomonas sp.)): capacity for plant growth promotion (using Poa annua seeds), Poa annua and Phragmites australis seed germination and...
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FY2017The Integrated Rangeland Fire Management Strategy Actionable Science Plan places a high priority on assessing control measures for invasive annual grasses, which provide fuel for rangeland fire and impede restoration of desirable perennials. Weed-suppressive strains of the bacterium Psuedomonas fluoresens (P.f.) are an emerging but yet untested tool for selectively reducing these annual grasses. P.f. may suppress annuals for approximately 2-5 years, bridging the short-term action of herbicides and long-term resistance provided as native bunchgrasses recover. In 2015-2016, the USGS responded to a request from the Idaho sage grouse action group to establish a series of field-based experiments to assess the efficacy...
map background search result map search result map Native and Invasive Species Plant Growth and Mortality in Growth Media Inoculated with Bacteria Found on Phragmites From New Jersey (2016) Evaluation of Weed-Suppressive Bacteria for Control of Exotic Annual Grasses Quantitative polymerase chain reaction (qPCR): An alternative, rapid water quality monitoring tool at a National Park on Lake Michigan. Modeling Escherichia coli in the Missouri River near Omaha, Nebraska, 2012-16: Model Inputs Streamwater constituent load data, models, and estimates for 15 watersheds in DeKalb County, Georgia, 2012–2016 Microbial Community Composition Data from Blacktail Creek near Williston, North Dakota Bacteria concentrations and microbial source indicators in the Middle Rio Grande collected by City of Albuquerque, Albuquerque, NM, 2018 Evaluation of Weed-Suppressive Bacteria for Control of Exotic Annual Grasses Quantitative polymerase chain reaction (qPCR): An alternative, rapid water quality monitoring tool at a National Park on Lake Michigan. Microbial Community Composition Data from Blacktail Creek near Williston, North Dakota Bacteria concentrations and microbial source indicators in the Middle Rio Grande collected by City of Albuquerque, Albuquerque, NM, 2018 Streamwater constituent load data, models, and estimates for 15 watersheds in DeKalb County, Georgia, 2012–2016 Modeling Escherichia coli in the Missouri River near Omaha, Nebraska, 2012-16: Model Inputs Native and Invasive Species Plant Growth and Mortality in Growth Media Inoculated with Bacteria Found on Phragmites From New Jersey (2016)