Filters: Tags: Bioassay (X) > Types: OGC WFS Layer (X)

Filters Date Range Past year (0) Past month (0) Past week (0) Past day (0) Custom range... To   Search Extensions Project (2) Types Map Service (3) OGC WMS Layer (3) OGC WMS Service (3) Contacts Edward F Bugliosi U.S. Environmental Protection Agency Barry P Baldigo Jennifer C Brennan New York State Department of Environmental Conservation More... Categories Project (2) Data (1) Tag Types Label (2) Theme (1) Place (1) Harvest Set (1) Tag Schemes https://www.sciencebase.gov/vocab/GLRI/keyword (2) https://www.sciencebase.gov/vocab/category/Great%20Lakes%20Restoration%20Initiative/SiGLProjectObjective (2) None (1) https://www.sciencebase.gov/vocab/GLRI/data-status (2) https://www.sciencebase.gov/vocab/category/Great%20Lakes%20Restoration%20Initiative/GLRIFocusArea (2) https://www.sciencebase.gov/vocab/category/Great%20Lakes%20Restoration%20Initiative/GLRILocationType (2) https://www.sciencebase.gov/vocab/category/Great%20Lakes%20Restoration%20Initiative/GLRITemplates (2) More... View Results as: JSON ATOM CSV

Benthic Communities and Sediment and Water Toxicity in the Rochester Embayment AOC Description of Work The study will be implemented in two phases due to logistical constraints and the need to incorporate methods developed (and findings) from a comparable investigation underway in another AOC. The first phase will consist of site selection, methods refinement, work-plan development, subcontract assembly, site reconnaissance, and sediment collection which will be done mainly during FY2013. The second phase will consist of macroinvertebrate identification, sediment toxicity testing, data analysis and interpretation, and report preparation and review mainly during FY2014. In brief, we will generate bed sediment toxicity and benthic community data needed to test two related hypotheses that address... Categories: Project; Types: Map Service, OGC WFS Layer, OGC WMS Layer, OGC WMS Service; Tags: Area of Concern, Areas of concern, Beneficial use impairment, Chironomus dilutus, GLRI, All tags... Genesee River, Great Lakes Restoration Initiative, Great Lakes Restoration Initiative, Lake, Lake:Lake Ontario, Rochester Embayment, Template 146, Toxic Substances, Uploaded in Batch, beneficial use impairment, bioassay, macroinvertebrate, plankton, sediment toxicity, Fewer tags Development of a dual luciferase-fluorescamine assay adapted to a 384 micro-well plate format-Data We have adapted two commonly used human breast luciferase transactivation cell bioassays, the recently re-named estrogen agonist/antagonist screening VM7Luc4E2 cell bioassay (previously designated BG1Luc4E2) and the androgen/glucocorticoid screening MDA-kb2 cell bioassay, to 384-well formats for HTS of endocrine-active substances. This cost-saving adaptation includes a fast, accurate, and easy measurement of protein amount in each well via the fluorescamine assay (in the form of relative fluorescence units, RFU which are then converted to microgram protein using bovine serum albumin standards) with which to normalize luciferase activity (in the form of relative light units, RLU) of cell lysates without requiring... Categories: Data; Types: Map Service, OGC WFS Layer, OGC WMS Layer, OGC WMS Service; Tags: Columbia Environmental Research Center, Missouri, USGS Science Data Catalog (SDC), bioassay, cells, All tags... fluorescence, luciferase, Fewer tags Benthic Communities and Sediment Toxicity in the St Lawrence River AOC Description of Work This study will generate bed sediment toxicity and benthic community data needed to test two related hypotheses that address the two criteria for delisting the benthos BUI. The first hypothesis is that bed sediments at selected sample locations in the AOC (in three tributaries and in the St. Lawrence River) are no more toxic to the test species than bed sediments collected from control sites located outside (generally upstream from) the AOC. Acute (survival) and chronic (growth) whole bed-sediment toxicity tests will be conducted using the midge (Chironomus dilutus), following standard methods (USEPA 1994; USEPA 2000). The second hypothesis is that the benthic macroinvertebrate communities from... Categories: Project; Types: Map Service, OGC WFS Layer, OGC WMS Layer, OGC WMS Service; Tags: Area of Concern, Areas of concern, Beneficial use impairment, Chironomus dilutus, GLRI, All tags... Great Lakes Restoration Initiative, Great Lakes Restoration Initiative, Lake, Lake:Lake Ontario, St. Lawrence River at Massena, Template 146, Toxic Substances, Uploaded in Batch, beneficial use impairment, bioassay, macroinvertebrate, sediment toxicity, Fewer tags