Filters: Tags: Environmental DNA (X) > partyWithName: Adam J Sepulveda (X)
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We developed and validated conventional and quantitative real-time PCR assays for the detection of DNA from the myxozoan parasite Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in fish. Assays were tested on fish tissue and on field-collected water samples to assess diagnostic and environmental DNA capabilities. The specificity, sensitivity, and broad applicability of the present assays surpass previous methods for detecting T. bryosalmonae DNA from fish tissue and water samples.
Positive and negative dreissenid mussel DNA quantitiative PCR results from environmental DNA water samples collected in Montana, Wisconsin and Minnesota to assess if environmental DNA can extend the seasonal window for dreissenid mussel early detection.
Categories: Data;
Tags: Flathead,
Missouri-Marias,
Pepin,
USGS Science Data Catalog (SDC),
Upper Mississippi,
Real-time PCR results of a round robin evaluation of 5 assays that target dreissenid mussel DNA. Water samples collected from waters with and without dreissenid mussels were analyzed using these five assays in four USGS laboratories. Samples from waters without dreissenid mussels were spiked with known amounts of dreissend DNA.
Field estimates of the abundance of rainbow trout in Washington and British Columbia were collected in concert with environmental DNA samples (eDNA) to evaluate if eDNA copy numbers correlated with abundance of trout. In addition, stream habitat data including channel units (pools, riffles), substrate, large woody debris, among others, were collected at sites.
Field estimates of the abundance of two trout species (bull trout and westslope cutthroat trout) in Montana and rainbow trout in Washington and British Columbia were collected in concert with environmental DNA samples (eDNA) to evaluate if eDNA copy numbers correlated with abundance of trout. In addition, stream habitat data including channel units (pools, riffles), substrate, large woody debris, among others, were collected at sites.
The dataset includes measurements of stream habitat, fish abundance of westslope cutthroat trout and bull trout, and species-specific measures of environmental DNA (eDNA) from within the water. The data covers multiple streams in western Montana.
U.S. Geological Survey decision analysts and technical experts worked with representatives from the U.S. Bureau of Reclamation, Utah Division of Wildlife Resources, and Central Utah Water Conservancy District to predict the consequences of alternative actions following environmental DNA detections of dreissenid mussel eDNA in Jordanelle Reservor, Utah. This dataset provides the consequence and model inputs for each of the alternative actions under different levels of uncertainty.
Categories: Data;
Tags: Aquatic Biology,
Ecology,
Genetics,
Jordanelle Reservoir,
USGS Science Data Catalog (SDC),
Environmental DNA results for March 2022 smallmouth bass surveillance in the Gardner River, Montana
Categories: Data;
Tags: Aquatic Biology,
Gardner River,
Montana,
Smallmouth Bass,
USGS Science Data Catalog (SDC),
U.S. Geological Survey decision analysts and technical experts worked with managers and stakeholders to predict the consequences of alternative actions following environmental DNA detections of invasive Najas minor eDNA in Sebago Lake, Maine, USA. This dataset provides the consequence and model inputs for each of the alternative actions under different levels of uncertainty.
Categories: Data;
Tags: Aquatic Biology,
Ecology,
Genetics,
Sebago Lake,
USGS Science Data Catalog (SDC),
Quantitative PCR data from mesocosm experiments and field evaluations comparing coarse filter-large water volume environmental DNA samples vs. fine filter-small water volume environmental DNA samples for detection and quantification of rainbow trout (Oncorhynchus mykiss) and bull trout (Salvelinus confluentus) DNA. Mesocosm experiments took place at the US Fish & Wildlife Service's Creston Fish Hatchery in Montana, 2016. Field surveys took place in the Flathead River basin of Montana, 2016.
The dataset contains qualitative detection and quantitative results of Esox Lucius DNA in environmental (e)DNA water samples collected from Miller Creek, Alaska and analyzed with quantitative PCR.
Categories: Data;
Tags: Alaska,
Aquatic Biology,
USGS Science Data Catalog (SDC),
biota,
environmental DNA
We tested the sensitivity of a portable, field-based environmental DNA (eDNA) platform relative to widely used lab-based eDNA approaches for detecting invasive northern pike (Esox lucius) in eight lakes on Alaska’s Kenai Peninsula. Raw data reported in this dataset report detect/non-detect data for technical replicates of water samples.
Categories: Data;
Tags: Alaska,
South Central Alaska,
USGS Science Data Catalog (SDC),
aquatic biology,
biota,
Environmental DNA results for water samples collected at USGS streamgages in the Columbia and Colorado river basins. Water samples were collected by USGS hydrologic technicians May - September 2018 and analyzed for presence of kokanee and yellow perch DNA. In this dataset, we report results and applicable covariates associated with each streamgage location. This is version 1.1 of this data release. The Kokanee_Perch_eDNA data and metadata files have been revised to correct for improper attribution of 16 samples. For access to previous versions, please contact the Point of Contact identified on this page.
Environmental DNA detection results from samples collected using autonomous water sampling robots and manual approaches. Samples were collected in the Upper Yellowstone River (Montana) and Upper Snake River (Idaho/Wyoming) in 2018 and 2019. Samples were tested for the DNA of the following species: the waterborne protozoa Naegleria spp., the fish pathogen Tetracapsuloides bryosalmonae, Scomber japonicas (mackerel fish), kokanee salmon (Oncorhynchus nerka) and dreissenid mussel (Dreissena spp.).
Categories: Data;
Tags: Palisades Dam,
USGS Science Data Catalog (SDC),
Upper Snake,
Upper Yellowstone,
Yellowstone National Park North,
Dreissenid mussel eDNA results for streamgage water samples in the Columbia and Colorado River basin
Environmental DNA results for water samples collected at USGS streamgages in the Columbia and Colorado river basins. Water samples were collected by USGS hydrologic technicians May - September 2018 and analyzed for presence of dreissenid mussel DNA. In this dataset, we report quantitative PCR results for each streamgage location.
Environmental DNA quantitative PCR results for water samples collected in 2018 at USGS streamgages downstream of Columbia River Basin reservoirs that are considered to have moderate to high suitability for invasive dreissenid mussels. Water samples were tested for DNA of dreissenid mussels, kokanee salmon, and yellow perch. This is version 1.1 of this data release. The Kokanee_Perch_eDNA data and metadata files have been revised to correct for improper attribution of 16 samples. For access to previous versions, please contact the Point of Contact identified on this page. First Posted March 29, 2019 Revised July 19, 2019, ver 1.1
Categories: Data,
Data Release - Revised;
Tags: Aquatic Biology,
Ecology,
Pacific Northwest,
Snake River,
USGS Science Data Catalog (SDC),
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