Filters: Tags: Environmental DNA (X) > partyWithName: Kylle A Roy (X)

Filters Date Range Past year (2) Past month (0) Past week (0) Past day (0) Custom range... To   Search Contacts Carter T Atkinson Pacific Island Ecosystems Research Center U.S. Geological Survey U.S. Geological Survey - ScienceBase Carolina Granthon Ecosystems Categories Data (7) Tag Types Theme (9) Place (9) USGS Scientific Topic Keyword (2) Harvest Set (2) Tag Schemes None (9) ISO 19115 Topic Category (9) View Results as: JSON ATOM CSV

Polyethylene Particle Detection (counts) during Environmental Sampler Comparison We designed two new samplers for monitoring airborne particulates, including fungal and fern spores and plant pollen, that rely on natural wind currents (Passive Environmental Sampler) or a battery operated fan (Active Environmental Sampler). Both samplers are modeled after commercial devices such as the Rotorod® and the Burkard samplers, but are more economical and require less maintenance than commercial devices. We conducted wind tunnel comparisons of our two new samplers to Rotorod® samplers using synthetic polyethylene spheres (12 - 160 µm in diameter) to compare numbers and size range of particulates that are captured by the samplers. This dataset contains raw numbers of polyethylene spheres that were captured... Categories: Data; Tags: Environmental DNA, Environmental Sampler, Hawaiii, Spore Traps, environment Orchidlands Estates Ceratocystis DNA Detection 2016 Rapid ʽŌhiʽa Death (ROD) currently threatens ōhiʽa lehua (Metrosideros polymorpha) on Hawaiʽi Island. First identified in Puna in 2014, the disease has now spread island wide. Besides direct sampling of trees, environmental sampling could serve as an easier and broader strategy to detect Ceratocystis spp., the fungi causing ROD. Environmental sampling could also help monitor the effect of felling ROD infected trees. We developed Passive and Active Environmental Samplers and deployed them at a property in Puna, where both C. lukuohia, and C. huliohia had been detected, and where the land owner practiced the management method of felling infected trees. We set up 2 Active Environmental Samplers (modified mosquito traps... Categories: Data; Tags: Ceratocystis, Environmental DNA, Environmental Sampler, Hawaiii, Management, All tags... Metrosideros polymorpha, Ohia lehua, ROD, Rapid Ohia Death, Spore Traps, environment, Fewer tags Ceratocystis lukuohia spore dilution for probit analysis Environmental DNA (eDNA) detection tools are becoming increasingly popular for documenting occurrence and distribution of native and invasive species. These tools can allow early detection of new diseases and invasive species and provide critical information for land management. We designed two new samplers for monitoring airborne particulates, including fungal and fern spores and plant pollen, that rely on natural wind currents (Passive Environmental Sampler) or a battery operated fan (Active Environmental Sampler). This dataset contains results of an experiment that was designed to determine probability of detecting known numbers of Ceratocystis lukuohia spores on individual slides in these samplers. Categories: Data; Tags: Ceratocystis, Environmental DNA, Environmental Sampler, Hawaiii, Metrosideros polymorpha, All tags... Ohia lehua, ROD, Rapid Ohia Death, Spore Traps, environment, Fewer tags Hawaii Island Environmental Sampler Comparison 2016-2018 These data include metadata and associated data files associated with the manuscript, "Economical Environmental Sampler Designs for Detecting Airborne Spread of Fungi Responsible for Rapid ʽŌhiʽa Death." These data include a total of 8 datasets used for both controlled and field studies evaluating the use of Active (with battery operated fan) and Passive (dependent on wind) USGS Environmental Samplers on Hawaii Island between 2016-2018. Samplers were operated under controlled laboratory and field conditions with a commercial sampler (Rotorod® Model 20) to compare efficacy in capturing synthetic polyethylene spheres (12 - 160 µm in diameter) and also Xyleborus spp. boring dust (frass) known to contain the fungi responsible... Tags: Ceratocystis, Ecology, Environmental DNA, Environmental Health, Environmental Sampler, All tags... Forestry, Genetics, Hawaiii, Metrosideros polymorpha, Ohia lehua, Rapid Ohia Death, Spore Traps, USGS Science Data Catalog (SDC), Xyleborus, environment, Fewer tags Ceratocystis DNA Detection in Frass During Caged Sampler Comparison We designed two new samplers for monitoring airborne particulates, including fungal and fern spores and plant pollen, that rely on natural wind currents (Passive Environmental Sampler) or a battery operated fan (Active Environmental Sampler). Both samplers are modeled after commercial devices such as the Rotorod® and the Burkard samplers, but are more economical and require less maintenance than commercial devices. We compared our two new samplers to Rotorod® samplers using Xyleborus spp. boring dust known to contain ROD causing pathogens. The comparison was done in a large outdoor field cage to determine relative effectiveness of the three samplers for capturing windblown boring dust. The dataset contains results... Categories: Data; Tags: Ceratocystis, Environmental DNA, Environmental Sampler, Hawaiii, Metrosideros polymorpha, All tags... Ohia lehua, ROD, Rapid Ohia Death, Spore Traps, environment, Fewer tags Sphere Particle Measurements During Sampler Comparison We designed two new samplers for monitoring airborne particulates, including fungal and fern spores and plant pollen, that rely on natural wind currents (Passive Environmental Sampler) or a battery operated fan (Active Environmental Sampler). Both samplers are modeled after commercial devices such as the Rotorod® and the Burkard samplers, but are more economical and require less maintenance than commercial devices. We conducted wind tunnel comparisons of our two new samplers to Rotorod® samplers using synthetic polyethylene spheres (12 - 160 µm in diameter) to compare numbers and size range of particulates that are captured by the samplers. This dataset contains raw particle sizes of polyethylene spheres that were... Categories: Data; Tags: Environmental DNA, Environmental Sampler, Hawaiii, Spore Traps, environment Frass Counts during Caged Sampler Comparison We designed two new samplers for monitoring airborne particulates, including fungal and fern spores and plant pollen, that rely on natural wind currents (Passive Environmental Sampler) or a battery operated fan (Active Environmental Sampler). Both samplers are modeled after commercial devices such as the Rotorod® and the Burkard samplers, but are more economical and require less maintenance than commercial devices. We compared our two new samplers to Rotorod® samplers using Xyleborus spp. boring dust (frass) known to contain fungi responsible for Rapid ʽŌhiʽa Death. The comparison was done in a large outdoor field cage to determine relative effectiveness of the three samplers for capturing windblown boring dust.... Categories: Data; Tags: Ceratocystis, Environmental DNA, Environmental DNA, Environmental Sampler, Environmental Sampler, All tags... Frass, Hawaiii, Metrosideros polymorpha, Metrosideros polymorpha, Ohia lehua, ROD, Rapid Ohia Death, Spore Traps, Spore Traps, Xyleborus, environment, Fewer tags Hawaii Island airborne detection of fungal pathogens of Ohia, 2016-2017 This data release includes metadata and tabular datasets that document (1) Austropuccina, Ceratocystis and Myrtaceae qPCR (quantitative polymerase chain reaction) DNA detections in Passive Environmental Samplers (PES), (2) wind speed, wind gust speed, and wind direction measurements collected at two sites in the Kahuku Unit of Hawai'i Volcanoes National Park (HAVO) where paired PES were located, (3) localities, sites and elevations where PES were located, and (4) Genbank accession numbers for Austropuccinia and Ceratocystis DNA sequences amplified from samples collected in a subset of PES. These raw data were analyzed and reported in the manuscript "Environmental Monitoring for Invasive Fungal Pathogens of ʽŌhiʽa... Tags: Austropuccinia psidii, Ceratocystis huliohia, Ceratocystis lukuohia, Ecology, Hawaii, All tags... Ohia Rust, Ohia lehua, Passive Environmental Sampler, Rapid Ohia Death, USGS Science Data Catalog (SDC), environment, environmental DNA, Fewer tags Frass Length Measurements during Caged Sampler Type Comparisons We designed two new samplers for monitoring airborne particulates, including fungal and fern spores and plant pollen, that rely on natural wind currents (Passive Environmental Sampler) or a battery operated fan (Active Environmental Sampler). Both samplers are modeled after commercial devices such as the Rotorod® and the Burkard samplers, but are more economical and require less maintenance than commercial devices. We compared our two new samplers to Rotorod® samplers using Xyleborus spp. boring dust (frass) known to contain fungi responsible for Rapid ʽŌhiʽa Death. The comparison was done in a large outdoor field cage to determine relative effectiveness of the three samplers for capturing windblown boring dust.... Categories: Data; Tags: Environmental DNA, Environmental Sampler, Frass, Hawaiii, Metrosideros polymorpha, All tags... ROD, Rapid Ohia Death, Spore Traps, Xyleborus, environment, Fewer tags