Environmental DNA (eDNA) Metabarcoding assessment of dead-end hollow fiber ultrafiltration (D-HFUF) and polyethylstyrene (PES) filters filtration methods on detection of freshwater mussel eDNA from Flint River and Spring Creek, Georgia and Big Piney River, Missouri

This dataset contains raw sequence data collected from an eDNA metabarcoding project to detect freshwater mussel species across two sites in Georgia (Spring Creek and Flint River) and one drainage in Missouri (Big Piney River). The eDNA samples were collected from each stream using dead-end ultra filtration (D-HFUF) with eDNA extracted from filters. We used two previously published primer sets designed to amplify freshwater mussels, one that amplified the mitochondrial cytochrome c oxidase I (COI) region and one that amplified the NADH dehydrogenase subunit I region, with negative control and mock community samples included. Samples were individually indexed, pooled, and sequenced on an Illumina MiSeq high-throughput...

Tags: Apalachicola, Aquatic Biology, Freshwater mussels, Gasconade-Osage, USGS Science Data Catalog (SDC), All tags... Unionidae, Wildlife Biology, dead-end hollow fiber ultrafiltration, environmental DNA, high-throughput sequencing, metabarcoding, Fewer tags