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ABSTRACT: The fungal pathogen Batrachochytrium dendrobatidis (Bd) causes chytridiomycosis, a disease implicated in amphibian declines on 5 continents. Polymerase chain reaction (PCR) primer sets exist with which amphibians can be tested for this disease, and advances in sampling techniques allow non-invasive testing of animals. We developed filtering and PCR based quantitative methods by modifying existing PCR assays to detect Bd DNA in water and sediments, without the need for testing amphibians; we tested the methods at 4 field sites. The SYBR based assay using Boyle primers (SYBR/Boyle assay) and the Taqman based assay using Wood primers performed similarly with samples generated in the laboratory (Bd spiked...
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This report is volume 2 of a two-volume ecological assessment of grassland ecosystems in the Southwestern United States. Broad-scale assessments are syntheses of current scientific knowledge, including a description of uncertainties and assumptions, to provide a characterization and comprehensive description of ecological, social, and economic components within an assessment area. Volume 1 of this assessment focused on the ecology, types, conditions, and management practices of Southwestern grasslands. Volume 2 (this volume) describes wildlife and fish species, their habitat requirements, and species-specific management concerns, in Southwestern grasslands. This assessment is regional in scale and pertains primarily...
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This report is volume 1 of a two-volume ecological assessment of grassland ecosystems in the Southwestern United States. Broadscale assessments are syntheses of current scientific knowledge, including a description of uncertainties and assumptions, to provide a characterization and comprehensive description of ecological, social, and economic components within an assessment area. Volume 1 of this assessment focuses on the ecology, types, conditions, and management practices of Southwestern grasslands. The second volume, due to be published in 2005, describes wildlife and fish species, their habitat requirements, and species-specific management concerns, in Southwestern grasslands. This assessment is regional in...
Highway runoff has the potential to negatively impact receiving systems including stormwater retention ponds where highway particulate matter can accumulate following runoff events. Tire wear particles, which contain about 1% Zn by mass, make up approximately one-third of the vehicle derived particulates in highway runoff and therefore may serve as a stressor to organisms utilizing retention ponds as habitat. In this study, we focused on the potential contribution of tire debris to Zn accumulation by Rana sylvatica larvae and possible lethal or sublethal impacts resulting from exposure to weathered tire debris during development. Eggs and larvae were exposed to aged sediments (containing either ZnCl2 or tire particulate...
Categories: Publication; Types: Citation; Tags: Amphibian, Tire, Toxicity, Zn, sediment


    map background search result map search result map Assessment of Grassland Ecosystem Conditions in the Southwestern United States, Volume 1 Assessment of Grassland Ecosystem Conditions in the Southwestern United States: Wildlife and Fish, Volume 2 Nevada Springs Conservation Plan Kentucky - Tier 1 Conservation Areas Kentucky - Tier 1 Conservation Areas Nevada Springs Conservation Plan Assessment of Grassland Ecosystem Conditions in the Southwestern United States, Volume 1 Assessment of Grassland Ecosystem Conditions in the Southwestern United States: Wildlife and Fish, Volume 2